3gr4: Difference between revisions

Revision as of 07:11, 1 November 2017

Activator-Bound Structure of Human Pyruvate Kinase M2

Structural highlights

3gr4 is a 4 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	ADP, DYY, FBP, TLA, UNX
Gene:	PKM2, PK2, PK3, PKM (HUMAN)
Activity:	Pyruvate kinase, with EC number 2.7.1.40
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[KPYM_HUMAN] Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.^[1] ^[2] ^[3]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

↑ Stetak A, Veress R, Ovadi J, Csermely P, Keri G, Ullrich A. Nuclear translocation of the tumor marker pyruvate kinase M2 induces programmed cell death. Cancer Res. 2007 Feb 15;67(4):1602-8. PMID:17308100 doi:10.1158/0008-5472.CAN-06-2870
↑ Lee J, Kim HK, Han YM, Kim J. Pyruvate kinase isozyme type M2 (PKM2) interacts and cooperates with Oct-4 in regulating transcription. Int J Biochem Cell Biol. 2008;40(5):1043-54. doi: 10.1016/j.biocel.2007.11.009., Epub 2007 Nov 29. PMID:18191611 doi:10.1016/j.biocel.2007.11.009
↑ Luo W, Hu H, Chang R, Zhong J, Knabel M, O'Meally R, Cole RN, Pandey A, Semenza GL. Pyruvate kinase M2 is a PHD3-stimulated coactivator for hypoxia-inducible factor 1. Cell. 2011 May 27;145(5):732-44. doi: 10.1016/j.cell.2011.03.054. PMID:21620138 doi:10.1016/j.cell.2011.03.054

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OCA

[1] Stetak A, Veress R, Ovadi J, Csermely P, Keri G, Ullrich A. Nuclear translocation of the tumor marker pyruvate kinase M2 induces programmed cell death. Cancer Res. 2007 Feb 15;67(4):1602-8. PMID:17308100 doi:10.1158/0008-5472.CAN-06-2870

[2] Lee J, Kim HK, Han YM, Kim J. Pyruvate kinase isozyme type M2 (PKM2) interacts and cooperates with Oct-4 in regulating transcription. Int J Biochem Cell Biol. 2008;40(5):1043-54. doi: 10.1016/j.biocel.2007.11.009., Epub 2007 Nov 29. PMID:18191611 doi:10.1016/j.biocel.2007.11.009

[3] Luo W, Hu H, Chang R, Zhong J, Knabel M, O'Meally R, Cole RN, Pandey A, Semenza GL. Pyruvate kinase M2 is a PHD3-stimulated coactivator for hypoxia-inducible factor 1. Cell. 2011 May 27;145(5):732-44. doi: 10.1016/j.cell.2011.03.054. PMID:21620138 doi:10.1016/j.cell.2011.03.054

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@@ Line 1: / Line 1: @@
 ==Activator-Bound Structure of Human Pyruvate Kinase M2==
 <StructureSection load='3gr4' size='340' side='right' caption='[[3gr4]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
@@ Line 6: / Line 7: @@
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PKM2, PK2, PK3, PKM ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pyruvate_kinase Pyruvate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.40 2.7.1.40] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gr4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gr4 OCA], [http://pdbe.org/3gr4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3gr4 RCSB], [http://www.ebi.ac.uk/pdbsum/3gr4 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gr4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gr4 OCA], [http://pdbe.org/3gr4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3gr4 RCSB], [http://www.ebi.ac.uk/pdbsum/3gr4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3gr4 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 47: / Line 48: @@
 [[Category: Thomas, C]]
 [[Category: Weigelt, J]]
+[[Category: Acetylation]]
 [[Category: Activator]]
 [[Category: Allosteric enzyme]]
+[[Category: Alternative splicing]]
 [[Category: Glycolysis]]
 [[Category: Kinase]]
@@ Line 54: / Line 57: @@
 [[Category: Metal-binding]]
 [[Category: Phosphoprotein]]
+[[Category: Polymorphism]]
 [[Category: Pyruvate]]
 [[Category: Sgc]]
 [[Category: Transferase]]

3gr4: Difference between revisions

Revision as of 07:11, 1 November 2017

Activator-Bound Structure of Human Pyruvate Kinase M2

Structural highlights

Function

Evolutionary Conservation

See Also

References

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