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'''STRUCTURAL CHARACTERISATION OF THE BROMOURACIL-GUANINE BASE PAIR MISMATCH IN A Z-DNA FRAGMENT''' | '''STRUCTURAL CHARACTERISATION OF THE BROMOURACIL-GUANINE BASE PAIR MISMATCH IN A Z-DNA FRAGMENT''' | ||
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==About this Structure== | ==About this Structure== | ||
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DA1 OCA]. | |||
==Reference== | ==Reference== | ||
Structural characterisation of the bromouracil.guanine base pair mismatch in a Z-DNA fragment., Brown T, Kneale G, Hunter WN, Kennard O, Nucleic Acids Res. 1986 Feb 25;14(4):1801-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/3951996 3951996] | Structural characterisation of the bromouracil.guanine base pair mismatch in a Z-DNA fragment., Brown T, Kneale G, Hunter WN, Kennard O, Nucleic Acids Res. 1986 Feb 25;14(4):1801-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/3951996 3951996] | ||
[[Category: Brown, T.]] | [[Category: Brown, T.]] | ||
[[Category: Hunter, W N.]] | [[Category: Hunter, W N.]] | ||
[[Category: Kennard, O.]] | [[Category: Kennard, O.]] | ||
[[Category: Kneale, G.]] | [[Category: Kneale, G.]] | ||
[[Category: | [[Category: Double helix]] | ||
[[Category: | [[Category: Mismatched]] | ||
[[Category: | [[Category: Modified]] | ||
[[Category: | [[Category: Z-dna]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 13:37:04 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 10:37, 2 May 2008
STRUCTURAL CHARACTERISATION OF THE BROMOURACIL-GUANINE BASE PAIR MISMATCH IN A Z-DNA FRAGMENT
Overview
The deoxyoligonucleotide d(BrU-G-C-G-C-G) was crystallised at pH 8.2 and its structure analysed by X-ray diffraction. The unit cell, of dimensions a = 17.94, b = 30.85, c = 49.94A contains four DNA duplexes in space group P2(1)2(1)2(1). The duplexes are in the Z conformation, with four Watson-Crick G.C base pairs and two BrU.G base pairs. The structure was refined to an R factor of 0.16 at a resolution of 2.2A with 64 solvent molecules located. The BrU.G base pair mismatch is of the wobble type, with both bases in the major tautomer form and hydrogen bonds linking 0-2 of BrU with N-1 of G and N3 of BrU with 0-6 of G. There is no indication of the presence of ionised base pairs, in spite of the high pH of crystallisation. The results are discussed in terms of the mutagenic properties of 5- bromouracil.
About this Structure
Full crystallographic information is available from OCA.
Reference
Structural characterisation of the bromouracil.guanine base pair mismatch in a Z-DNA fragment., Brown T, Kneale G, Hunter WN, Kennard O, Nucleic Acids Res. 1986 Feb 25;14(4):1801-9. PMID:3951996 Page seeded by OCA on Fri May 2 13:37:04 2008
