2hup: Difference between revisions

Revision as of 08:16, 18 October 2017

Crystal structure of human RAB43 in complex with GDP

Structural highlights

2hup is a 2 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	GDP, MG, UNX
Gene:	RAB43 (HUMAN)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[RAB43_HUMAN] The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. The low intrinsic GTPase activity of RAB43 is activated by USP6NL. Involved in retrograde transport from the endocytic pathway to the Golgi apparatus. Involved in the transport of Shiga toxin from early and recycling endosomes to the trans-Golgi network. Required for the structural integrity of the Golgi complex. Plays a role in the maturation of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis.^[1] ^[2] ^[3] ^[4]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

↑ Fuchs E, Haas AK, Spooner RA, Yoshimura S, Lord JM, Barr FA. Specific Rab GTPase-activating proteins define the Shiga toxin and epidermal growth factor uptake pathways. J Cell Biol. 2007 Jun 18;177(6):1133-43. Epub 2007 Jun 11. PMID:17562788 doi:https://dx.doi.org/10.1083/jcb.200612068
↑ Haas AK, Yoshimura S, Stephens DJ, Preisinger C, Fuchs E, Barr FA. Analysis of GTPase-activating proteins: Rab1 and Rab43 are key Rabs required to maintain a functional Golgi complex in human cells. J Cell Sci. 2007 Sep 1;120(Pt 17):2997-3010. Epub 2007 Aug 7. PMID:17684057 doi:https://dx.doi.org/10.1242/jcs.014225
↑ Dejgaard SY, Murshid A, Erman A, Kizilay O, Verbich D, Lodge R, Dejgaard K, Ly-Hartig TB, Pepperkok R, Simpson JC, Presley JF. Rab18 and Rab43 have key roles in ER-Golgi trafficking. J Cell Sci. 2008 Aug 15;121(Pt 16):2768-81. doi: 10.1242/jcs.021808. Epub 2008, Jul 29. PMID:18664496 doi:https://dx.doi.org/10.1242/jcs.021808
↑ Seto S, Tsujimura K, Koide Y. Rab GTPases regulating phagosome maturation are differentially recruited to mycobacterial phagosomes. Traffic. 2011 Apr;12(4):407-20. doi: 10.1111/j.1600-0854.2011.01165.x. Epub 2011 , Feb 21. PMID:21255211 doi:10.1111/j.1600-0854.2011.01165.x

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OCA

[1] Fuchs E, Haas AK, Spooner RA, Yoshimura S, Lord JM, Barr FA. Specific Rab GTPase-activating proteins define the Shiga toxin and epidermal growth factor uptake pathways. J Cell Biol. 2007 Jun 18;177(6):1133-43. Epub 2007 Jun 11. PMID:17562788 doi:https://dx.doi.org/10.1083/jcb.200612068

[2] Haas AK, Yoshimura S, Stephens DJ, Preisinger C, Fuchs E, Barr FA. Analysis of GTPase-activating proteins: Rab1 and Rab43 are key Rabs required to maintain a functional Golgi complex in human cells. J Cell Sci. 2007 Sep 1;120(Pt 17):2997-3010. Epub 2007 Aug 7. PMID:17684057 doi:https://dx.doi.org/10.1242/jcs.014225

[3] Dejgaard SY, Murshid A, Erman A, Kizilay O, Verbich D, Lodge R, Dejgaard K, Ly-Hartig TB, Pepperkok R, Simpson JC, Presley JF. Rab18 and Rab43 have key roles in ER-Golgi trafficking. J Cell Sci. 2008 Aug 15;121(Pt 16):2768-81. doi: 10.1242/jcs.021808. Epub 2008, Jul 29. PMID:18664496 doi:https://dx.doi.org/10.1242/jcs.021808

[4] Seto S, Tsujimura K, Koide Y. Rab GTPases regulating phagosome maturation are differentially recruited to mycobacterial phagosomes. Traffic. 2011 Apr;12(4):407-20. doi: 10.1111/j.1600-0854.2011.01165.x. Epub 2011 , Feb 21. PMID:21255211 doi:10.1111/j.1600-0854.2011.01165.x

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@@ Line 1: / Line 1: @@
 ==Crystal structure of human RAB43 in complex with GDP==
 <StructureSection load='2hup' size='340' side='right' caption='[[2hup]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene></td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">RAB43 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hup FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hup OCA], [http://pdbe.org/2hup PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2hup RCSB], [http://www.ebi.ac.uk/pdbsum/2hup PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hup FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hup OCA], [http://pdbe.org/2hup PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2hup RCSB], [http://www.ebi.ac.uk/pdbsum/2hup PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2hup ProSAT]</span></td></tr>
 </table>
 == Function ==

2hup: Difference between revisions

Revision as of 08:16, 18 October 2017

Crystal structure of human RAB43 in complex with GDP

Structural highlights

Function

Evolutionary Conservation

References

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