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'''COCRYSTAL STRUCTURE OF THE MESSENGER RNA 5' CAP-BINDING PROTEIN (EIF4E) BOUND TO 7-METHYL-GDP''' | '''COCRYSTAL STRUCTURE OF THE MESSENGER RNA 5' CAP-BINDING PROTEIN (EIF4E) BOUND TO 7-METHYL-GDP''' | ||
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[[Category: Marcotrigiano, J.]] | [[Category: Marcotrigiano, J.]] | ||
[[Category: Sonenberg, N.]] | [[Category: Sonenberg, N.]] | ||
[[Category: | [[Category: Protein 5' mrna cap complex]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:09:35 2008'' | |||
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Revision as of 12:09, 2 May 2008
COCRYSTAL STRUCTURE OF THE MESSENGER RNA 5' CAP-BINDING PROTEIN (EIF4E) BOUND TO 7-METHYL-GDP
Overview
The X-ray structure of the eukaryotic translation initiation factor 4E (eIF4E), bound to 7-methyl-GDP, has been determined at 2.2 A resolution. eIF4E recognizes 5' 7-methyl-G(5')ppp(5')N mRNA caps during the rate-limiting initiation step of translation. The protein resembles a cupped hand and consists of a curved, 8-stranded antiparallel beta sheet, backed by three long alpha helices. 7-methyl-GDP binds in a narrow cap-binding slot on the molecule's concave surface, where 7-methyl-guanine recognition is mediated by base sandwiching between two conserved tryptophans, plus formation of three hydrogen bonds and a van der Waals contact between its N7-methyl group and a third conserved tryptophan. The convex dorsal surface of the molecule displays a phylogenetically conserved hydrophobic/acidic portion, which may interact with other translation initiation factors and regulatory proteins.
About this Structure
1EJ1 is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
Cocrystal structure of the messenger RNA 5' cap-binding protein (eIF4E) bound to 7-methyl-GDP., Marcotrigiano J, Gingras AC, Sonenberg N, Burley SK, Cell. 1997 Jun 13;89(6):951-61. PMID:9200613 Page seeded by OCA on Fri May 2 15:09:35 2008
