1esb: Difference between revisions

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[[Image:1esb.gif|left|200px]]
[[Image:1esb.gif|left|200px]]


{{Structure
<!--
|PDB= 1esb |SIZE=350|CAPTION= <scene name='initialview01'>1esb</scene>, resolution 2.3&Aring;
The line below this paragraph, containing "STRUCTURE_1esb", creates the "Structure Box" on the page.
|SITE=
You may change the PDB parameter (which sets the PDB file loaded into the applet)
|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PHQ:FORMIC+ACID+BENZYL+ESTER'>PHQ</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Pancreatic_elastase Pancreatic elastase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.36 3.4.21.36] </span>
or leave the SCENE parameter empty for the default display.
|GENE=  
-->
|DOMAIN=
{{STRUCTURE_1esb| PDB=1esb  | SCENE= }}  
|RELATEDENTRY=
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1esb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1esb OCA], [http://www.ebi.ac.uk/pdbsum/1esb PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1esb RCSB]</span>
}}


'''DIRECT STRUCTURE OBSERVATION OF AN ACYL-ENZYME INTERMEDIATE IN THE HYDROLYSIS OF AN ESTER SUBSTRATE BY ELASTASE'''
'''DIRECT STRUCTURE OBSERVATION OF AN ACYL-ENZYME INTERMEDIATE IN THE HYDROLYSIS OF AN ESTER SUBSTRATE BY ELASTASE'''
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[[Category: Rasmussen, B.]]
[[Category: Rasmussen, B.]]
[[Category: Ringe, D.]]
[[Category: Ringe, D.]]
[[Category: hydrolase(serine proteinase)]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 15:27:51 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:08:35 2008''

Revision as of 12:27, 2 May 2008

File:1esb.gif

Template:STRUCTURE 1esb

DIRECT STRUCTURE OBSERVATION OF AN ACYL-ENZYME INTERMEDIATE IN THE HYDROLYSIS OF AN ESTER SUBSTRATE BY ELASTASE


Overview

The method of X-ray crystallographic cryoenzymology has been used to determine the crystal structure of a kinetically significant species on the reaction pathway of a crystalline enzyme. The structure of a specific acyl-enzyme intermediate in the elastase-catalyzed hydrolysis of the N-carbobenzoxy-L-alanine p-nitrophenyl ester has been determined and refined against X-ray diffraction data at 2.3-A resolution. The difference Fourier electron density map clearly shows electron density for the trapped acyl-enzyme. The acyl-enzyme was formed at -26 degrees C and was stabilized at -55 degrees C during data collection, taking advantage of the glass transition in protein dynamics that occurs at around -50 degrees C.

About this Structure

1ESB is a Single protein structure of sequence from Sus scrofa. Full crystallographic information is available from OCA.

Reference

Direct structural observation of an acyl-enzyme intermediate in the hydrolysis of an ester substrate by elastase., Ding X, Rasmussen BF, Petsko GA, Ringe D, Biochemistry. 1994 Aug 9;33(31):9285-93. PMID:8049229 Page seeded by OCA on Fri May 2 15:27:51 2008

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