3h6f

Crystal Structure of Mycobacterium Tuberculosis Proteasome Modified by inhibitor HT1171

spinqualitylabelsall models 3h6f, resolution 2.51Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3h6f is a 28 chain structure with sequence from "bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: DMF NonStd Res: OZT Related: 2fhg, 2fhh, 3h6i Gene: prcA, Rv2109c ("Bacillus tuberculosis" (Zopf 1883) Klein 1884), MT2170, prcB, Rv2110c ("Bacillus tuberculosis" (Zopf 1883) Klein 1884) Activity: Proteasome endopeptidase complex, with EC number 3.4.25.1 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [PSA_MYCTO] Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation.[HAMAP-Rule:MF_00289] [PSB_MYCTO] Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed Many anti-infectives inhibit the synthesis of bacterial proteins, but none selectively inhibits their degradation. Most anti-infectives kill replicating pathogens, but few preferentially kill pathogens that have been forced into a non-replicating state by conditions in the host. To explore these alternative approaches we sought selective inhibitors of the proteasome of Mycobacterium tuberculosis. Given that the proteasome structure is extensively conserved, it is not surprising that inhibitors of all chemical classes tested have blocked both eukaryotic and prokaryotic proteasomes, and no inhibitor has proved substantially more potent on proteasomes of pathogens than of their hosts. Here we show that certain oxathiazol-2-one compounds kill non-replicating M. tuberculosis and act as selective suicide-substrate inhibitors of the M. tuberculosis proteasome by cyclocarbonylating its active site threonine. Major conformational changes protect the inhibitor-enzyme intermediate from hydrolysis, allowing formation of an oxazolidin-2-one and preventing regeneration of active protease. Residues outside the active site whose hydrogen bonds stabilize the critical loop before and after it moves are extensively non-conserved. This may account for the ability of oxathiazol-2-one compounds to inhibit the mycobacterial proteasome potently and irreversibly while largely sparing the human homologue. Inhibitors selective for mycobacterial versus human proteasomes.,Lin G, Li D, de Carvalho LP, Deng H, Tao H, Vogt G, Wu K, Schneider J, Chidawanyika T, Warren JD, Li H, Nathan C Nature. 2009 Oct 1;461(7264):621-6. Epub 2009 Sep 16. PMID:19759536 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Proteasome

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