1brl

THREE-DIMENSIONAL STRUCTURE OF BACTERIAL LUCIFERASE FROM VIBRIO HARVEYI AT 2.4 ANGSTROMS RESOLUTION

spinqualitylabelsall models 1brl, resolution 2.40Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 1brl is a 4 chain structure with sequence from Vibrio harveyi. The June 2006 RCSB PDB Molecule of the Month feature on Luciferase by David S. Goodsell is 10.2210/rcsb_pdb/mom_2006_6. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: PO4 Activity: Alkanal monooxygenase (FMN-linked), with EC number 1.14.14.3 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [LUXA_VIBHA] Light-emitting reaction in luminous bacteria. [LUXB_VIBHA] Light-emitting reaction in luminous bacteria. The specific role of the beta subunit is unknown, but it is absolutely required for bioluminescence activity. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed Luciferases are a class of enzymes that generate light in the visible spectrum. Luciferase from luminous marine bacteria is an alpha-beta heterodimer monooxygenase that catalyzes the oxidation of FMNH2 and a long-chain aliphatic aldehyde. The X-ray crystal structure of bacterial luciferase from Vibrio harveyi has been determined to 2.4 A resolution. The structure was solved by a combination of multiple isomorphous replacement and molecular averaging between the two heterodimers in the asymmetric unit. Each subunit folds into a (beta/alpha)8 barrel motif, and dimerization is mediated through a parallel four-helix bundle centered on a pseudo 2-fold axis that relates the structurally similar subunits. The vicinity of the active site has been identified on the alpha subunit by correlations with similar protein motifs and previous biochemical studies. The structure presented here represents the first molecular model of a bioluminescent enzyme. Three-dimensional structure of bacterial luciferase from Vibrio harveyi at 2.4 A resolution.,Fisher AJ, Raushel FM, Baldwin TO, Rayment I Biochemistry. 1995 May 23;34(20):6581-6. PMID:7756289 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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