1g7r: Difference between revisions
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{{STRUCTURE_1g7r| PDB=1g7r | SCENE= }} | |||
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'''X-RAY STRUCTURE OF TRANSLATION INITIATION FACTOR IF2/EIF5B''' | '''X-RAY STRUCTURE OF TRANSLATION INITIATION FACTOR IF2/EIF5B''' | ||
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[[Category: Dever, T E.]] | [[Category: Dever, T E.]] | ||
[[Category: Roll-Mecak, A.]] | [[Category: Roll-Mecak, A.]] | ||
[[Category: | [[Category: Translational gtpase]] | ||
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Revision as of 14:14, 2 May 2008
X-RAY STRUCTURE OF TRANSLATION INITIATION FACTOR IF2/EIF5B
Overview
X-ray structures of the universal translation initiation factor IF2/eIF5B have been determined in three states: free enzyme, inactive IF2/eIF5B.GDP, and active IF2/eIF5B.GTP. The "chalice-shaped" enzyme is a GTPase that facilitates ribosomal subunit joining and Met-tRNA(i) binding to ribosomes in all three kingdoms of life. The conserved core of IF2/eIF5B consists of an N-terminal G domain (I) plus an EF-Tu-type beta barrel (II), followed by a novel alpha/beta/alpha-sandwich (III) connected via an alpha helix to a second EF-Tu-type beta barrel (IV). Structural comparisons reveal a molecular lever, which amplifies a modest conformational change in the Switch 2 region of the G domain induced by Mg(2+)/GTP binding over a distance of 90 A from the G domain active center to domain IV. Mechanisms of GTPase function and ribosome binding are discussed.
About this Structure
1G7R is a Single protein structure of sequence from Methanothermobacter thermautotrophicus. Full crystallographic information is available from OCA.
Reference
X-Ray structures of the universal translation initiation factor IF2/eIF5B: conformational changes on GDP and GTP binding., Roll-Mecak A, Cao C, Dever TE, Burley SK, Cell. 2000 Nov 22;103(5):781-92. PMID:11114334 Page seeded by OCA on Fri May 2 17:14:55 2008
