Introduction to Evolutionary Conservation: Difference between revisions
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===Sophisticated Analysis of Conservation: ConSurf=== | ===Sophisticated Analysis of Conservation: ConSurf=== | ||
The above analysis assigns each amino acid in enolase to one of three categories: conserved, similar, or different. This is very simplistic. In contrast, the analysis used in Proteopedia (and in the above image) is sophisticated, using many more sequences, and weighting the impact of each sequence in the multiple sequence alignment according to the phylogenetic tree calculated from the alignment. This sophisticated determination of conservation and variability is done by the [[ConSurfDB vs. ConSurf|ConSurf Servers]] (see also summaries of their mechanism [http://www.umass.edu/molvis/workshop/slides/consurf3.htm short], or [[ConSurfDB_vs._ConSurf#The_ConSurf-DB_Mechanism|longer]]). ConSurf divides conservation into 9 levels, and colors them as follows: | The above analysis assigns each amino acid in enolase to one of three categories: conserved, similar, or different. This is very simplistic. In contrast, the analysis used in Proteopedia (and in the above image) is sophisticated, using many more sequences, and weighting the impact of each sequence in the multiple sequence alignment according to the phylogenetic tree calculated from the alignment. This sophisticated determination of conservation and variability is done by the [[ConSurfDB vs. ConSurf|ConSurf Servers]] (see also summaries of their mechanism: [http://www.umass.edu/molvis/workshop/slides/consurf3.htm short version], or [[ConSurfDB_vs._ConSurf#The_ConSurf-DB_Mechanism|longer version]]). ConSurf divides conservation into 9 levels, and colors them as follows: | ||
<center>{{Template:ColorKey_ConSurf_NoGray}}</center> | <center>{{Template:ColorKey_ConSurf_NoGray}}</center> | ||
