Sandbox Reserved 427: Difference between revisions

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'''The Vitamin D Binding Site'''

The Vitamin D binding site is located in domain I and contains helices 1 through 6. The binding site is lined with hydrophobic residues that interact with the hydrophobic parts of the vitamin D3 ligand.<scene name='48/483884/Site_where_25ohd3_binds/3'>Hydrogen Bonds</scene> are formed between, the 25-hydroxyl and Tyr 32 with a distance of 2.85 angstroms, the 3-hydroxyl and Ser 76 with a distance of 3.01 angstroms and Met 109 with a distance of 3.01 angstroms.[1] Different analogs of the vitamin D3 ligand influence hydrogen bond locations and binding affinities. <scene name='48/483884/Jy_site_where_25ohd3_binds/5'>The JY analog</scene>, for example, has a binding affinity to DBP of 1314, which is much greater compared to the affinity of 25OHD3, which is 667.[1] This is due to the <scene name='48/483884/Stacking_stabilization_with_jy/3'>stacking of the aromatic sidechain of JY</scene> and the aromatic residues of Phe 24, Tyr 34, Phe 36, and Tyr 38. The JX analog switches the meta hydroxyl group on JY to para, increasing the binding affinity to 2111. By switching the hydroxyl group to the para position tighter hydrogen bonds can be formed to the Ser 28 residue stabilizing the complex.[1]

The Vitamin D binding site is located in domain I and contains helices 1 through 6. The binding site is lined with hydrophobic residues that interact with the hydrophobic parts of the vitamin D3 ligand.<scene name='48/483884/Site_where_25ohd3_binds/3'>Hydrogen Bonds</scene>(locations highlighted in yellow) are formed between, the 25-hydroxyl and Tyr 32 with a distance of 2.85 angstroms, the 3-hydroxyl and Ser 76 with a distance of 3.01 angstroms and Met 109 with a distance of 3.01 angstroms.[1] Different analogs of the vitamin D3 ligand influence hydrogen bond locations and binding affinities. <scene name='48/483884/Jy_site_where_25ohd3_binds/5'>The JY analog</scene>(yellow), for example, has a binding affinity to DBP of 1314, which is much greater compared to the affinity of 25OHD3, which is 667.[1] This is due to the <scene name='48/483884/Stacking_stabilization_with_jy/3'>stacking of the aromatic sidechain of JY</scene> and the aromatic residues of Phe 24, Tyr 34, Phe 36, and Tyr 38. The JX analog switches the meta hydroxyl group on JY to para, increasing the binding affinity to 2111. By switching the hydroxyl group to the para position tighter hydrogen bonds can be formed to the Ser 28 residue stabilizing the complex.[1]

'''Biological Relevance of The Vitamin D Binding Site'''

Sandbox Reserved 427: Difference between revisions

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@@ Line 35: / Line 35: @@
 '''The Vitamin D Binding Site'''
-The Vitamin D binding site is located in domain I and contains helices 1 through 6. The binding site is lined with hydrophobic residues that interact with the hydrophobic parts of the vitamin D3 ligand.<scene name='48/483884/Site_where_25ohd3_binds/3'>Hydrogen Bonds</scene> are formed between, the 25-hydroxyl and Tyr 32 with a distance of 2.85 angstroms, the 3-hydroxyl and Ser 76 with a distance of 3.01 angstroms and Met 109 with a distance of 3.01 angstroms.[1] Different analogs of the vitamin D3 ligand influence hydrogen bond locations and binding affinities. <scene name='48/483884/Jy_site_where_25ohd3_binds/5'>The JY analog</scene>, for example, has a binding affinity to DBP of 1314, which is much greater compared to the affinity of 25OHD3, which is 667.[1] This is due to the <scene name='48/483884/Stacking_stabilization_with_jy/3'>stacking of the aromatic sidechain of JY</scene> and the aromatic residues of Phe 24, Tyr 34, Phe 36, and Tyr 38. The JX analog switches the meta hydroxyl group on JY to para, increasing the binding affinity to 2111. By switching the hydroxyl group to the para position tighter hydrogen bonds can be formed to the Ser 28 residue stabilizing the complex.[1]
+The Vitamin D binding site is located in domain I and contains helices 1 through 6. The binding site is lined with hydrophobic residues that interact with the hydrophobic parts of the vitamin D3 ligand.<scene name='48/483884/Site_where_25ohd3_binds/3'>Hydrogen Bonds</scene>(locations highlighted in yellow) are formed between, the 25-hydroxyl and Tyr 32 with a distance of 2.85 angstroms, the 3-hydroxyl and Ser 76 with a distance of 3.01 angstroms and Met 109 with a distance of 3.01 angstroms.[1] Different analogs of the vitamin D3 ligand influence hydrogen bond locations and binding affinities. <scene name='48/483884/Jy_site_where_25ohd3_binds/5'>The JY analog</scene>(yellow), for example, has a binding affinity to DBP of 1314, which is much greater compared to the affinity of 25OHD3, which is 667.[1] This is due to the <scene name='48/483884/Stacking_stabilization_with_jy/3'>stacking of the aromatic sidechain of JY</scene> and the aromatic residues of Phe 24, Tyr 34, Phe 36, and Tyr 38. The JX analog switches the meta hydroxyl group on JY to para, increasing the binding affinity to 2111. By switching the hydroxyl group to the para position tighter hydrogen bonds can be formed to the Ser 28 residue stabilizing the complex.[1]
 '''Biological Relevance of The Vitamin D Binding Site'''