Sandbox Reserved 1177: Difference between revisions
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===Active State=== | ===Active State=== | ||
After determining <scene name='72/721547/Ntsr1-gw5/8'>NTSR1-GW5</scene> as only active-like, research was conducted to determine the structure of NTSR1 with catalytic nucleotide exchange. In order to do so, three of the six mutations were reverted back <ref name="SPGP"/>, and these three residues were selected on the basis of their location. The reversion of E166A, L310A, and F358A led to NTSR1 with G-protein activity at almost wild-type level. This protein was named, <scene name='72/721548/Ntsr1-elf/ | After determining <scene name='72/721547/Ntsr1-gw5/8'>NTSR1-GW5</scene> as only active-like, research was conducted to determine the structure of NTSR1 with catalytic nucleotide exchange. In order to do so, three of the six mutations were reverted back <ref name="SPGP"/>, and these three residues were selected on the basis of their location. The reversion of E166A, L310A, and F358A led to NTSR1 with G-protein activity at almost wild-type level. This protein was named, <scene name='72/721548/Ntsr1-elf/6'>NTSR1-ELF</scene>, and indicated that the amino acid residues E166, L310, and F358 play significant roles in the activity of NTSR1 <ref name="SPGP"/>. | ||
====Leu310==== | ====Leu310==== | ||
