Sandbox Reserved 1176: Difference between revisions

Line 40:

====Glu166====

Although the role of <scene name='72/721548/E166/4'>Glu166</scene> in G-protein activity is not quite as clear as it is for <scene name='72/721548/L310/3'>Leu310</scene> or <scene name='72/721547/Hydrophobic_binding_pocket/5'>Phe358</scene>, substituting this residue for an alanine significantly reduced '''[https://en.wikipedia.org/wiki/Active_site catalytic]''' G-protein activity <ref name="SPGP"/>. Glu166 is part of a <scene name='72/721548/Dery_motif/2'>D/ERY motif</scene> that is highly conserved in class A GPCRs and includes Arg167 and Tyr168. To determine a role for Glu166, other class A GPCRs were structurally analyzed. It's hypothesized <ref name="SPGP"/> that E166 in NTSR1 interacts with Val102, Thr101,and His105 to stabilize the G protein. The possibility of an important connection between the D/ERY motif and intracellular loop 2, ~~which~~ plays a role in the dissociation of the receptor-G protein complex with GTP present ~~has also been noted.<ref name="SPGP"/>~~ M181 is believed to link the D/ERY motif and ICL2. <ref name="SPGP"/>

Although the role of <scene name='72/721548/E166/4'>Glu166</scene> in G-protein activity is not quite as clear as it is for <scene name='72/721548/L310/3'>Leu310</scene> or <scene name='72/721547/Hydrophobic_binding_pocket/5'>Phe358</scene>, substituting this residue for an alanine significantly reduced '''[https://en.wikipedia.org/wiki/Active_site catalytic]''' G-protein activity <ref name="SPGP"/>. Glu166 is part of a <scene name='72/721548/Dery_motif/2'>D/ERY motif</scene> that is highly conserved in class A GPCRs and includes Arg167 and Tyr168. To determine a role for Glu166, other class A GPCRs were structurally analyzed. It's hypothesized <ref name="SPGP"/> that E166 in NTSR1 interacts with Val102, Thr101,and His105 to stabilize the G protein. The possibility of an important connection between the D/ERY motif and intracellular loop 2, has also been noted.<ref name="SPGP"/>. ICL2 plays a role in the dissociation of the receptor-G protein complex with GTP present, and M181 is believed to link the D/ERY motif and ICL2.<ref name="SPGP"/>

Sandbox Reserved 1176: Difference between revisions

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

Revision as of 02:10, 22 April 2016 view source Danny Cotter (talk \| contribs) 188 edits No edit summary ← Older edit		Revision as of 02:13, 22 April 2016 view source Danny Cotter (talk \| contribs) 188 edits No edit summary Newer edit →
Line 40:		Line 40:
	====Glu166====		====Glu166====

	Although the role of <scene name='72/721548/E166/4'>Glu166</scene> in G-protein activity is not quite as clear as it is for <scene name='72/721548/L310/3'>Leu310</scene> or <scene name='72/721547/Hydrophobic_binding_pocket/5'>Phe358</scene>, substituting this residue for an alanine significantly reduced '''[https://en.wikipedia.org/wiki/Active_site catalytic]''' G-protein activity <ref name="SPGP"/>. Glu166 is part of a <scene name='72/721548/Dery_motif/2'>D/ERY motif</scene> that is highly conserved in class A GPCRs and includes Arg167 and Tyr168. To determine a role for Glu166, other class A GPCRs were structurally analyzed. It's hypothesized <ref name="SPGP"/> that E166 in NTSR1 interacts with Val102, Thr101,and His105 to stabilize the G protein. The possibility of an important connection between the D/ERY motif and intracellular loop 2, ~~which~~ plays a role in the dissociation of the receptor-G protein complex with GTP present ~~has also been noted.<ref name="SPGP"/>~~ M181 is believed to link the D/ERY motif and ICL2. <ref name="SPGP"/>		Although the role of <scene name='72/721548/E166/4'>Glu166</scene> in G-protein activity is not quite as clear as it is for <scene name='72/721548/L310/3'>Leu310</scene> or <scene name='72/721547/Hydrophobic_binding_pocket/5'>Phe358</scene>, substituting this residue for an alanine significantly reduced '''[https://en.wikipedia.org/wiki/Active_site catalytic]''' G-protein activity <ref name="SPGP"/>. Glu166 is part of a <scene name='72/721548/Dery_motif/2'>D/ERY motif</scene> that is highly conserved in class A GPCRs and includes Arg167 and Tyr168. To determine a role for Glu166, other class A GPCRs were structurally analyzed. It's hypothesized <ref name="SPGP"/> that E166 in NTSR1 interacts with Val102, Thr101,and His105 to stabilize the G protein. The possibility of an important connection between the D/ERY motif and intracellular loop 2, has also been noted.<ref name="SPGP"/>. ICL2 plays a role in the dissociation of the receptor-G protein complex with GTP present, and M181 is believed to link the D/ERY motif and ICL2.<ref name="SPGP"/>