5iwo: Difference between revisions

Revision as of 07:20, 10 June 2020

Bacterial sodium channel pore domain, low bromide

Structural highlights

5iwo is a 4 chain structure with sequence from Atcc baa-1101. This structure supersedes the now removed PDB entry 5hku. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	BR
Related:	5hj8, 5hk6, 5hk7, 5hkd, 5iwn
Gene:	Mlg_0322 (ATCC BAA-1101)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

Voltage-gated ion channels (VGICs) are outfitted with diverse cytoplasmic domains that impact function. To examine how such elements may affect VGIC behavior, we addressed how the bacterial voltage-gated sodium channel (BacNaV) C-terminal cytoplasmic domain (CTD) affects function. Our studies show that the BacNaV CTD exerts a profound influence on gating through a temperature-dependent unfolding transition in a discrete cytoplasmic domain, the neck domain, proximal to the pore. Structural and functional studies establish that the BacNaV CTD comprises a bi-partite four-helix bundle that bears an unusual hydrophilic core whose integrity is central to the unfolding mechanism and that couples directly to the channel activation gate. Together, our findings define a general principle for how the widespread four-helix bundle cytoplasmic domain architecture can control VGIC responses, uncover a mechanism underlying the diverse BacNaV voltage dependencies, and demonstrate that a discrete domain can encode the temperature-dependent response of a channel.

Unfolding of a Temperature-Sensitive Domain Controls Voltage-Gated Channel Activation.,Arrigoni C, Rohaim A, Shaya D, Findeisen F, Stein RA, Nurva SR, Mishra S, Mchaourab HS, Minor DL Jr Cell. 2016 Feb 25;164(5):922-36. doi: 10.1016/j.cell.2016.02.001. PMID:26919429^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Arrigoni C, Rohaim A, Shaya D, Findeisen F, Stein RA, Nurva SR, Mishra S, Mchaourab HS, Minor DL Jr. Unfolding of a Temperature-Sensitive Domain Controls Voltage-Gated Channel Activation. Cell. 2016 Feb 25;164(5):922-36. doi: 10.1016/j.cell.2016.02.001. PMID:26919429 doi:https://dx.doi.org/10.1016/j.cell.2016.02.001

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OCA

[1] Arrigoni C, Rohaim A, Shaya D, Findeisen F, Stein RA, Nurva SR, Mishra S, Mchaourab HS, Minor DL Jr. Unfolding of a Temperature-Sensitive Domain Controls Voltage-Gated Channel Activation. Cell. 2016 Feb 25;164(5):922-36. doi: 10.1016/j.cell.2016.02.001. PMID:26919429 doi:https://dx.doi.org/10.1016/j.cell.2016.02.001

[1]

@@ Line 1: / Line 1: @@
 ==Bacterial sodium channel pore domain, low bromide==
-<StructureSection load='5iwo' size='340' side='right' caption='[[5iwo]], [[Resolution|resolution]] 3.33&Aring;' scene=''>
+<StructureSection load='5iwo' size='340' side='right'caption='[[5iwo]], [[Resolution|resolution]] 3.33&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5iwo]] is a 4 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=5hku 5hku]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IWO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5IWO FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5iwo]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_baa-1101 Atcc baa-1101]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=5hku 5hku]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IWO OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5IWO FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5hj8|5hj8]], [[5hk6|5hk6]], [[5hk7|5hk7]], [[5hkd|5hkd]], [[5iwn|5iwn]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5iwo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5iwo OCA], [http://pdbe.org/5iwo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5iwo RCSB], [http://www.ebi.ac.uk/pdbsum/5iwo PDBsum]</span></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Mlg_0322 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=351052 ATCC BAA-1101])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5iwo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5iwo OCA], [http://pdbe.org/5iwo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5iwo RCSB], [http://www.ebi.ac.uk/pdbsum/5iwo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5iwo ProSAT]</span></td></tr>
 </table>
 <div style="background-color:#fffaf0;">
@@ Line 21: / Line 22: @@
 __TOC__
 </StructureSection>
+[[Category: Atcc baa-1101]]
+[[Category: Large Structures]]
 [[Category: Findeisen, F]]
 [[Category: Minor, D L]]

5iwo: Difference between revisions

Revision as of 07:20, 10 June 2020

Bacterial sodium channel pore domain, low bromide

Structural highlights

Publication Abstract from PubMed

References

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