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==Synthetic Applications==

Recent advances in programmable site-directed DNA-binding proteins (such as CRISPR-[[Cas9]]) have shown incredible potential for medical, agricultural, and scientific ~~application~~. As a result, it is not surprising that researchers are attempting to develop similar protein-based tools for programmable RNA binding. Unfortunately, few RNA-binding proteins act in a manner which is predictable enough to facilitate convenient RNA manipulation. PPR proteins hold great promise for this application. As described previously, PPR proteins bind RNA nucleotides in a specific and predictable manner, so researchers are attempting to develop custom-made PPR proteins for use in manipulative gene expression experiments.

Recent advances in programmable site-directed DNA-binding proteins (such as CRISPR-[[Cas9]]) have shown incredible potential for medical, agricultural, and scientific applications.<ref>doi:10.1126/science.1231143</ref> As a result, it is not surprising that researchers are attempting to develop similar protein-based tools for programmable RNA binding. Unfortunately, few RNA-binding proteins act in a manner which is predictable enough to facilitate convenient RNA manipulation. PPR proteins hold great promise for this application. As described previously, PPR proteins bind RNA nucleotides in a specific and predictable manner, so researchers are attempting to develop custom-made PPR proteins for use in manipulative gene expression experiments.

===Limitations===

Sandbox WWC7: Difference between revisions

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 ==Synthetic Applications==
-Recent advances in programmable site-directed DNA-binding proteins (such as CRISPR-[[Cas9]]) have shown incredible potential for medical, agricultural, and scientific application. As a result, it is not surprising that researchers are attempting to develop similar protein-based tools for programmable RNA binding. Unfortunately, few RNA-binding proteins act in a manner which is predictable enough to facilitate convenient RNA manipulation. PPR proteins hold great promise for this application. As described previously, PPR proteins bind RNA nucleotides in a specific and predictable manner, so researchers are attempting to develop custom-made PPR proteins for use in manipulative gene expression experiments.
+Recent advances in programmable site-directed DNA-binding proteins (such as CRISPR-[[Cas9]]) have shown incredible potential for medical, agricultural, and scientific applications.<ref>doi:10.1126/science.1231143</ref> As a result, it is not surprising that researchers are attempting to develop similar protein-based tools for programmable RNA binding. Unfortunately, few RNA-binding proteins act in a manner which is predictable enough to facilitate convenient RNA manipulation. PPR proteins hold great promise for this application. As described previously, PPR proteins bind RNA nucleotides in a specific and predictable manner, so researchers are attempting to develop custom-made PPR proteins for use in manipulative gene expression experiments.
 ===Limitations===