5f8b: Difference between revisions
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<StructureSection load='5f8b' size='340' side='right' caption='[[5f8b]], [[Resolution|resolution]] 2.54Å' scene=''> | <StructureSection load='5f8b' size='340' side='right' caption='[[5f8b]], [[Resolution|resolution]] 2.54Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5f8b]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5F8B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5F8B FirstGlance]. <br> | <table><tr><td colspan='2'>[[5f8b]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspfu Aspfu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5F8B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5F8B FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene>, <scene name='pdbligand=PZS:(5~{S},8~{S},9~{R})-2-[(~{E})-HEX-1-ENYL]-8-METHOXY-3-METHYL-9-OXIDANYL-8-(PHENYLCARBONYL)-1-OXA-7-AZASPIRO[4.4]NON-2-ENE-4,6-DIONE'>PZS</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene>, <scene name='pdbligand=PZS:(5~{S},8~{S},9~{R})-2-[(~{E})-HEX-1-ENYL]-8-METHOXY-3-METHYL-9-OXIDANYL-8-(PHENYLCARBONYL)-1-OXA-7-AZASPIRO[4.4]NON-2-ENE-4,6-DIONE'>PZS</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5f8b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5f8b OCA], [http://pdbe.org/5f8b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5f8b RCSB], [http://www.ebi.ac.uk/pdbsum/5f8b PDBsum]</span></td></tr> | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">AFUA_8G00580 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=330879 ASPFU])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5f8b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5f8b OCA], [http://pdbe.org/5f8b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5f8b RCSB], [http://www.ebi.ac.uk/pdbsum/5f8b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5f8b ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Geometric isomerization can expand the scope of biological activities of natural products. The observed chemical diversity among the pseurotin-type fungal secondary metabolites is in part generated by a trans to cis isomerization of an olefin. In vitro characterizations of pseurotin biosynthetic enzymes revealed that the glutathione S-transferase PsoE requires participation of the bifunctional C-methyltransferase/epoxidase PsoF to complete the trans to cis isomerization of the pathway intermediate presynerazol. The crystal structure of the PsoE/glutathione/presynerazol complex indicated stereospecific glutathione-presynerazol conjugate formation is the principal function of PsoE. Moreover, PsoF was identified to have an additional, unexpected oxidative isomerase activity, thus making it a trifunctional enzyme which is key to the complexity generation in pseurotin biosynthesis. Through the study, we identified a novel mechanism of accomplishing a seemingly simple trans to cis isomerization reaction. | |||
Oxidative trans to cis Isomerization of Olefins in Polyketide Biosynthesis.,Yamamoto T, Tsunematsu Y, Hara K, Suzuki T, Kishimoto S, Kawagishi H, Noguchi H, Hashimoto H, Tang Y, Hotta K, Watanabe K Angew Chem Int Ed Engl. 2016 May 17;55(21):6207-10. doi: 10.1002/anie.201600940. , Epub 2016 Apr 13. PMID:27072782<ref>PMID:27072782</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 5f8b" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Aspfu]] | |||
[[Category: Hara, K]] | [[Category: Hara, K]] | ||
[[Category: Hashimoto, H]] | [[Category: Hashimoto, H]] | ||
