1idp: Difference between revisions
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'''Crystal structure of scytalone dehydratase F162A mutant in the unligated state''' | '''Crystal structure of scytalone dehydratase F162A mutant in the unligated state''' | ||
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[[Category: Nakasako, M.]] | [[Category: Nakasako, M.]] | ||
[[Category: Yamaguchi, I.]] | [[Category: Yamaguchi, I.]] | ||
[[Category: | [[Category: Lyase]] | ||
[[Category: | [[Category: Melanine biosynthesis]] | ||
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Revision as of 16:53, 2 May 2008
Crystal structure of scytalone dehydratase F162A mutant in the unligated state
Overview
Scytalone dehydratase variant F162A, in which Phe162 in the C-terminal region was replaced with alanine, was crystallized with polyethylene glycol 4000. Because the crystal was radiation-sensitive, the diffraction data were collected at cryogenic temperatures. The crystal belonged to monoclinic space group P2(1), with unit-cell parameters a = 72.64, b = 61.30, c = 72.62 A, beta = 120.02 degrees at 37 K. The calculated V(M) value was acceptable when a trimer of the mutant enzyme occupied a crystallographic asymmetric unit. The resolution limit was extended to 1.45 A at BL41XU of SPring-8 at 37 K.
About this Structure
1IDP is a Single protein structure of sequence from Magnaporthe grisea. Full crystallographic information is available from OCA.
Reference
Crystallization of scytalone dehydratase F162A mutant in the unligated state and a preliminary X-ray diffraction study at 37 K., Motoyama T, Nakasako M, Yamaguchi I, Acta Crystallogr D Biol Crystallogr. 2002 Jan;58(Pt 1):148-50. Epub 2001, Dec 21. PMID:11752795 Page seeded by OCA on Fri May 2 19:53:07 2008
