Nitrite reductase: Difference between revisions
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Protein film voltammetry (PFV) experiments performed on ''S. oneidensis'' ccNiR films in the absence of substrate produced a broad envelope of reversible signals that span approximately 450 mV. At high pH values the envelope appears as a single peak, whereas at pH values below 7 the envelope appears to be composed of two large overlapping peaks. At pH values below 6 and at 0 °C, the envelope of signal can be better resolved and more than two peaks can be observed. This resulting envelope of signal can be deconvoluted as the sum of five one-electron peaks, each corresponding to one of the five hemes in a ccNiR monomer (see image below). | Protein film voltammetry (PFV) experiments performed on ''S. oneidensis'' ccNiR films in the absence of substrate produced a broad envelope of reversible signals that span approximately 450 mV. At high pH values the envelope appears as a single peak, whereas at pH values below 7 the envelope appears to be composed of two large overlapping peaks. At pH values below 6 and at 0 °C, the envelope of signal can be better resolved and more than two peaks can be observed. This resulting envelope of signal can be deconvoluted as the sum of five one-electron peaks, each corresponding to one of the five hemes in a ccNiR monomer (see image below). | ||
[[Image:figur5.jpg|left|378px|thumb|PFV of ''S. oneidensis'' ccNiR (a) Typical signal on a graphite electrode. (b) Baselinesubtracted non-turnover voltammogram]] | [[Image:figur5.jpg|left|378px|thumb|PFV of ''S. oneidensis'' ccNiR (a) Typical signal on a graphite electrode. (b) Baselinesubtracted non-turnover voltammogram]] | ||
