5ina: Difference between revisions

Line 1:

~~'''Unreleased structure'''~~

~~The entry~~ 5ina is ~~ON HOLD~~ ~~until Paper Publication~~

==Chaperone Spy bound to Im7 6-45 ensemble==

~~Authors~~:

== Structural highlights ==

<table><tr><td colspan='2'>[[5ina]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5INA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5INA FirstGlance]. <br>

~~Description~~:

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>

[[Category: ~~Unreleased Structures~~]]

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ina FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ina OCA], [http://pdbe.org/5ina PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ina RCSB], [http://www.ebi.ac.uk/pdbsum/5ina PDBsum]</span></td></tr>

</table>

== Function ==

[[http://www.uniprot.org/uniprot/SPY_ECOLI SPY_ECOLI]] An ATP-independent periplasmic chaperone, decreases protein aggregation and helps protein refolding. Binds substrate over a large region of its convex inner surface (PubMed:21317898, PubMed:24497545). Substrate protein folds while it is bound to chaperone (PubMed:26619265). Increasing Spy flexibility increases its substrate affinity and overall chaperone activity (shown for 3 different substrates) (PubMed:24497545). Protects proteins in vitro against tannin inactivation; tannins have antimicrobial activity (PubMed:21317898). Overexpression enhances the stability of otherwise unstable periplasmic proteins (PubMed:21317898).<ref>PMID:21317898</ref> <ref>PMID:24497545</ref> <ref>PMID:26619265</ref> [[http://www.uniprot.org/uniprot/IMM7_ECOLX IMM7_ECOLX]] This protein is able to protect a cell, which harbors the plasmid ColE7 encoding colicin E7, against colicin E7, it binds specifically to the DNase-type colicin and inhibits its bactericidal activity. Dimeric ImmE7 may possess a RNase activity that cleaves its own mRNA at a specific site and thus autoregulates translational expression of the downstream ceiE7 gene as well as degradation of the upstream ceaE7 mRNA.

== References ==

__TOC__

</StructureSection>

[[Category: Afonine, P V]]

[[Category: Ahlstrom, L S]]

[[Category: Bardwell, J C.A]]

[[Category: Brooks, C L]]

[[Category: Horowitz, S]]

[[Category: Koldewey, P]]

[[Category: Martin, R]]

[[Category: Salmon, L]]

[[Category: Trievel, R C]]

[[Category: Xu, Q]]

[[Category: Chaperone]]

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 5ina is ON HOLD  until Paper Publication
+==Chaperone Spy bound to Im7 6-45 ensemble==
+<StructureSection load='5ina' size='340' side='right' caption='[[5ina]], [[Resolution|resolution]] 1.79&Aring;' scene=''>
-Authors:
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5ina]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5INA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5INA FirstGlance]. <br>
-Description:
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-[[Category: Unreleased Structures]]
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ina FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ina OCA], [http://pdbe.org/5ina PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ina RCSB], [http://www.ebi.ac.uk/pdbsum/5ina PDBsum]</span></td></tr>
+</table>
+== Function ==
+[[http://www.uniprot.org/uniprot/SPY_ECOLI SPY_ECOLI]] An ATP-independent periplasmic chaperone, decreases protein aggregation and helps protein refolding. Binds substrate over a large region of its convex inner surface (PubMed:21317898, PubMed:24497545). Substrate protein folds while it is bound to chaperone (PubMed:26619265). Increasing Spy flexibility increases its substrate affinity and overall chaperone activity (shown for 3 different substrates) (PubMed:24497545). Protects proteins in vitro against tannin inactivation; tannins have antimicrobial activity (PubMed:21317898). Overexpression enhances the stability of otherwise unstable periplasmic proteins (PubMed:21317898).<ref>PMID:21317898</ref> <ref>PMID:24497545</ref> <ref>PMID:26619265</ref>  [[http://www.uniprot.org/uniprot/IMM7_ECOLX IMM7_ECOLX]] This protein is able to protect a cell, which harbors the plasmid ColE7 encoding colicin E7, against colicin E7, it binds specifically to the DNase-type colicin and inhibits its bactericidal activity. Dimeric ImmE7 may possess a RNase activity that cleaves its own mRNA at a specific site and thus autoregulates translational expression of the downstream ceiE7 gene as well as degradation of the upstream ceaE7 mRNA.
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Afonine, P V]]
+[[Category: Ahlstrom, L S]]
+[[Category: Bardwell, J C.A]]
+[[Category: Brooks, C L]]
+[[Category: Horowitz, S]]
+[[Category: Koldewey, P]]
+[[Category: Martin, R]]
+[[Category: Salmon, L]]
+[[Category: Trievel, R C]]
+[[Category: Xu, Q]]
+[[Category: Chaperone]]