5j4f: Difference between revisions
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==Crystal structure of the N-terminally His6-tagged HP0902, an uncharacterized protein from Helicobacter pylori 26695== | |||
<StructureSection load='5j4f' size='340' side='right' caption='[[5j4f]], [[Resolution|resolution]] 1.40Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5j4f]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J4F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5J4F FirstGlance]. <br> | |||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5j4f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j4f OCA], [http://pdbe.org/5j4f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5j4f RCSB], [http://www.ebi.ac.uk/pdbsum/5j4f PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5j4f ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
We solved the crystal structure of a functionally uncharacterized protein, HP0902, from Helicobacter pylori. Its structure demonstrated an all-beta cupin fold that cannot bind metal ions due to the absence of a metal-binding histidine that is conserved in many metallo-cupins. In contrast, isothermal titration calorimetry and NMR titration demonstrated that HP0902 is able to bind bacterial endotoxin lipopolysaccharides (LPS) through its surface-exposed loops, where metal-binding sites are usually found in other metallo-cupins. This report constitutes the first identification of an LPS-interacting protein, both in the cupin family and in H. pylori. Furthermore, identification of the ability of HP0902 to bind LPS uncovers a putative role for this protein in H. pylori pathogenicity. | |||
Structural identification of the lipopolysaccharide-binding capability of a cupin-family protein from Helicobacter pylori.,Sim DW, Kim JH, Kim HY, Jang JH, Lee WC, Kim EH, Park PJ, Lee KH, Won HS FEBS Lett. 2016 Sep;590(17):2997-3004. doi: 10.1002/1873-3468.12332. Epub 2016, Aug 11. PMID:27466800<ref>PMID:27466800</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 5j4f" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Kim, H Y]] | |||
[[Category: Kim, J H]] | |||
[[Category: Lee, W C]] | |||
[[Category: Sim, D W]] | |||
[[Category: Won, H S]] | |||
[[Category: Cupin family]] | |||
[[Category: Helicobacter pylori]] | |||
[[Category: Hp0902]] | |||
[[Category: Secretory protein]] | |||
[[Category: Uncharacterized protein]] | |||
[[Category: Unknown function]] | |||
Revision as of 09:24, 10 March 2017
Crystal structure of the N-terminally His6-tagged HP0902, an uncharacterized protein from Helicobacter pylori 26695
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