5j4g: Difference between revisions

Line 1:

~~'''Unreleased structure'''~~

~~The entry~~ 5j4g is ~~ON HOLD until Paper~~ Publication

==Crystal structure of the C-terminally His6-tagged HP0902, an uncharacterized protein from Helicobacter pylori 26695==

== Structural highlights ==

<table><tr><td colspan='2'>[[5j4g]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J4G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5J4G FirstGlance]. <br>

</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5j4f|5j4f]]</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5j4g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j4g OCA], [http://pdbe.org/5j4g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5j4g RCSB], [http://www.ebi.ac.uk/pdbsum/5j4g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5j4g ProSAT]</span></td></tr>

</table>

== Publication Abstract from PubMed ==

We solved the crystal structure of a functionally uncharacterized protein, HP0902, from Helicobacter pylori. Its structure demonstrated an all-beta cupin fold that cannot bind metal ions due to the absence of a metal-binding histidine that is conserved in many metallo-cupins. In contrast, isothermal titration calorimetry and NMR titration demonstrated that HP0902 is able to bind bacterial endotoxin lipopolysaccharides (LPS) through its surface-exposed loops, where metal-binding sites are usually found in other metallo-cupins. This report constitutes the first identification of an LPS-interacting protein, both in the cupin family and in H. pylori. Furthermore, identification of the ability of HP0902 to bind LPS uncovers a putative role for this protein in H. pylori pathogenicity.

~~Authors~~:

Structural identification of the lipopolysaccharide-binding capability of a cupin-family protein from Helicobacter pylori.,Sim DW, Kim JH, Kim HY, Jang JH, Lee WC, Kim EH, Park PJ, Lee KH, Won HS FEBS Lett. 2016 Sep;590(17):2997-3004. doi: 10.1002/1873-3468.12332. Epub 2016, Aug 11. PMID:27466800<ref>PMID:27466800</ref>

~~Description~~:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

[[Category: ~~Unreleased Structures~~]]

</div>

== References ==

__TOC__

</StructureSection>

[[Category: Kim, H Y]]

[[Category: Kim, J H]]

[[Category: Lee, W C]]

[[Category: Sim, D W]]

[[Category: Won, H S]]

[[Category: Cupin family]]

[[Category: Helicobacter pylori]]

[[Category: Hp0902]]

[[Category: Secretory protein]]

[[Category: Uncharacterized protein]]

[[Category: Unknown function]]

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 5j4g is ON HOLD  until Paper Publication
+==Crystal structure of the C-terminally His6-tagged HP0902, an uncharacterized protein from Helicobacter pylori 26695==
+<StructureSection load='5j4g' size='340' side='right' caption='[[5j4g]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5j4g]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J4G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5J4G FirstGlance]. <br>
+</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5j4f|5j4f]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5j4g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j4g OCA], [http://pdbe.org/5j4g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5j4g RCSB], [http://www.ebi.ac.uk/pdbsum/5j4g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5j4g ProSAT]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+We solved the crystal structure of a functionally uncharacterized protein, HP0902, from Helicobacter pylori. Its structure demonstrated an all-beta cupin fold that cannot bind metal ions due to the absence of a metal-binding histidine that is conserved in many metallo-cupins. In contrast, isothermal titration calorimetry and NMR titration demonstrated that HP0902 is able to bind bacterial endotoxin lipopolysaccharides (LPS) through its surface-exposed loops, where metal-binding sites are usually found in other metallo-cupins. This report constitutes the first identification of an LPS-interacting protein, both in the cupin family and in H. pylori. Furthermore, identification of the ability of HP0902 to bind LPS uncovers a putative role for this protein in H. pylori pathogenicity.
-Authors:
+Structural identification of the lipopolysaccharide-binding capability of a cupin-family protein from Helicobacter pylori.,Sim DW, Kim JH, Kim HY, Jang JH, Lee WC, Kim EH, Park PJ, Lee KH, Won HS FEBS Lett. 2016 Sep;590(17):2997-3004. doi: 10.1002/1873-3468.12332. Epub 2016, Aug 11. PMID:27466800<ref>PMID:27466800</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 5j4g" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Kim, H Y]]
+[[Category: Kim, J H]]
+[[Category: Lee, W C]]
+[[Category: Sim, D W]]
+[[Category: Won, H S]]
+[[Category: Cupin family]]
+[[Category: Helicobacter pylori]]
+[[Category: Hp0902]]
+[[Category: Secretory protein]]
+[[Category: Uncharacterized protein]]
+[[Category: Unknown function]]