5g56: Difference between revisions
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==THE TETRA-MODULAR CELLULOSOMAL ARABINOXYLANASE CtXyl5A STRUCTURE AS REVEALED BY X-RAY CRYSTALLOGRAPHY== | |||
<StructureSection load='5g56' size='340' side='right' caption='[[5g56]], [[Resolution|resolution]] 2.64Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5g56]] is a 1 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=5ak1 5ak1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G56 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5G56 FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr> | |||
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5g56 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g56 OCA], [http://pdbe.org/5g56 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5g56 RCSB], [http://www.ebi.ac.uk/pdbsum/5g56 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5g56 ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The cellulosome, a highly elaborate extracellular multi-enzyme complex of cellulases and hemicellulases, is responsible for the degradation of plant cell walls. The xylanase CtXyl5A (Cthe_2193) is a multimodular arabinoxylanase which is one of the largest components of the Clostridium thermocellum cellulosome. The N-terminal catalytic domain of CtXyl5A, which is a member of glycoside hydrolase family 5 (GH5), is responsible for the hydrolysis of arabinoxylans. Appended after it are three noncatalytic carbohydrate-binding modules (CBMs), which belong to families 6 (CBM6), 13 (CBM13) and 62 (CBM62). In addition, CtXyl5A has a fibronectin type III-like (Fn3) module preceding the CBM62 and a type I dockerin (DOK) module following it which allows the enzyme to be integrated into the cellulosome through binding to a cohesin module of the protein scaffold CipA. Crystals of the pentamodular enzyme without the DOK module at the C-terminus, with the domain architecture CtGH5-CBM6-CBM13-Fn3-CBM62, have been obtained. The structure of this pentamodular xylanase has been determined by molecular replacement to a resolution of 2.64 A using coordinates of CtGH5-CBM6, Fn3 and CBM62 from the PDB as search models. | |||
Purification, crystallization and preliminary X-ray characterization of the pentamodular arabinoxylanase CtXyl5A from Clostridium thermocellum.,Bras JL, Correia MA, Romao MJ, Prates JA, Fontes CM, Najmudin S Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Jul 1;67(Pt 7):833-6. doi:, 10.1107/S1744309111020823. Epub 2011 Jun 30. PMID:21795807<ref>PMID:21795807</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 5g56" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Bras, J L.A]] | |||
[[Category: Ferreira, L M.A]] | |||
[[Category: Fontes, C M.G A]] | |||
[[Category: Gilbert, H J]] | |||
[[Category: Najmudin, S]] | [[Category: Najmudin, S]] | ||
[[Category: | [[Category: Arabinoxylanase]] | ||
[[Category: | [[Category: Carbohydrate binding protein]] | ||
[[Category: Cbm13]] | |||
[[Category: Cbm6]] | |||
[[Category: Cellulosome]] | |||
[[Category: Clostridium thermocellum]] | |||
[[Category: Ctxyl5a]] | |||
[[Category: Fn3]] | |||
[[Category: Gh5]] | |||
Revision as of 17:28, 12 July 2016
THE TETRA-MODULAR CELLULOSOMAL ARABINOXYLANASE CtXyl5A STRUCTURE AS REVEALED BY X-RAY CRYSTALLOGRAPHY
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