5b6q: Difference between revisions
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==Crystal structure of monomeric cytochrome c5 from Shewanella violacea== | |||
<StructureSection load='5b6q' size='340' side='right' caption='[[5b6q]], [[Resolution|resolution]] 1.78Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5b6q]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5B6Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5B6Q FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5b6q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5b6q OCA], [http://pdbe.org/5b6q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5b6q RCSB], [http://www.ebi.ac.uk/pdbsum/5b6q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5b6q ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Monomeric cytochrome c5 from deep-sea piezophilic Shewanella violacea (SVcytc5) was stable against heat and denaturant compared with the homologous protein from shallow-sea piezo-sensitive Shewanella livingstonensis (SLcytc5). Here, the SVcytc5 crystal structure revealed that the Lys-50 side chain on the flexible loop formed a hydrogen bond with heme whereas that of corresponding hydrophobic Leu-50 could not form such a bond in SLcytc5, which appeared to be one of possible factors responsible for the difference in stability between the two proteins. This structural insight was confirmed by a reciprocal mutagenesis study on the thermal stability of these two proteins. As SVcytc5 was isolated from a deep-sea piezophilic bacterium, the present comparative study indicates that adaptation of monomeric SVcytc5 to high pressure environments results in stabilization against heat. | |||
Comparative study on stabilization mechanism of monomeric cytochrome c5 from deep-sea piezophilic Shewanella violacea.,Masanari M, Fujii S, Kawahara K, Oki H, Tsujino H, Maruno T, Kobayashi Y, Ohkubo T, Wakai S, Sambongi Y Biosci Biotechnol Biochem. 2016 Dec;80(12):2365-2370. Epub 2016 Sep 20. PMID:27648635<ref>PMID:27648635</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 5b6q" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Fujii, S]] | |||
[[Category: Harada, Y]] | |||
[[Category: Kawahara, K]] | |||
[[Category: Kobayashi, Y]] | |||
[[Category: Maruno, T]] | |||
[[Category: Masanari, M]] | |||
[[Category: Nishiyama, M]] | |||
[[Category: Ohkubo, T]] | |||
[[Category: Oki, H]] | |||
[[Category: Sambongi, Y]] | |||
[[Category: Tsujino, H]] | |||
[[Category: Wakai, S]] | |||
[[Category: Cytochrome c5]] | |||
[[Category: Electron transport]] | |||
[[Category: Protein stability]] | |||
[[Category: Shewanella]] | |||
