5lem: Difference between revisions
From Proteopedia
Jump to navigationJump to search
m Protected "5lem" [edit=sysop:move=sysop] |
No edit summary |
||
| Line 1: | Line 1: | ||
==Crystal structure of DARPin-DARPin rigid fusion, variant DD_Off7_11_3G124 in complex with Maltose-binding Protein and Green Fluorescent Protein== | |||
<StructureSection load='5lem' size='340' side='right' caption='[[5lem]], [[Resolution|resolution]] 2.98Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5lem]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeqvi Aeqvi], [http://en.wikipedia.org/wiki/Eco57 Eco57] and [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LEM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LEM FirstGlance]. <br> | |||
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene></td></tr> | |||
[[Category: | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">malE, Z5632, ECs5017 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 ECO57]), GFP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6100 AEQVI])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lem FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lem OCA], [http://pdbe.org/5lem PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lem RCSB], [http://www.ebi.ac.uk/pdbsum/5lem PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lem ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[[http://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI]] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin. [[http://www.uniprot.org/uniprot/MALE_ECO57 MALE_ECO57]] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides (By similarity). | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Aeqvi]] | |||
[[Category: Eco57]] | |||
[[Category: Synthetic construct sequences]] | |||
[[Category: Batyuk, A]] | |||
[[Category: Mittl, P R]] | |||
[[Category: Plueckthun, A]] | |||
[[Category: Wu, Y]] | |||
[[Category: Designed ankyrin repeat protein]] | |||
[[Category: Fluorescent protein]] | |||
[[Category: Protein design]] | |||
[[Category: Protein engineering]] | |||
[[Category: Rigid domain fusion]] | |||
[[Category: X-ray crystallography]] | |||
Revision as of 08:00, 13 November 2017
Crystal structure of DARPin-DARPin rigid fusion, variant DD_Off7_11_3G124 in complex with Maltose-binding Protein and Green Fluorescent Protein
| ||||||||||||