5kve: Difference between revisions

Line 1:

~~'''Unreleased structure'''~~

~~The entry~~ 5kve is ~~ON HOLD~~

==Zika specific antibody, ZV-48, bound to ZIKA envelope DIII==

== Structural highlights ==

<table><tr><td colspan='2'>[[5kve]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KVE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KVE FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>

<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5kvd|5kvd]], [[5kvf|5kvf]], [[5kvg|5kvg]]</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kve FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kve OCA], [http://pdbe.org/5kve PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kve RCSB], [http://www.ebi.ac.uk/pdbsum/5kve PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kve ProSAT]</span></td></tr>

</table>

== Publication Abstract from PubMed ==

Zika virus (ZIKV) infection during pregnancy has emerged as a global public health problem because of its ability to cause severe congenital disease. Here, we developed six mouse monoclonal antibodies (mAbs) against ZIKV including four (ZV-48, ZV-54, ZV-64, and ZV-67) that were ZIKV specific and neutralized infection of African, Asian, and American strains to varying degrees. X-ray crystallographic and competition binding analyses of Fab fragments and scFvs defined three spatially distinct epitopes in DIII of the envelope protein corresponding to the lateral ridge (ZV-54 and ZV-67), C-C' loop (ZV-48 and ZV-64), and ABDE sheet (ZV-2) regions. In vivo passive transfer studies revealed protective activity of DIII-lateral ridge specific neutralizing mAbs in a mouse model of ZIKV infection. Our results suggest that DIII is targeted by multiple type-specific antibodies with distinct neutralizing activity, which provides a path for developing prophylactic antibodies for use in pregnancy or designing epitope-specific vaccines against ZIKV.

~~Authors:~~ Zhao, H., Nelson, ~~C.A.~~, Fremont~~, D~~.H.~~, Center for Structural Genomics of Infectious Diseases~~ (~~CSGID~~)

Structural Basis of Zika Virus-Specific Antibody Protection.,Zhao H, Fernandez E, Dowd KA, Speer SD, Platt DJ, Gorman MJ, Govero J, Nelson CA, Pierson TC, Diamond MS, Fremont DH Cell. 2016 Jul 26. pii: S0092-8674(16)30927-8. doi: 10.1016/j.cell.2016.07.020. PMID:27475895<ref>PMID:27475895</ref>

~~Description: Zika specific antibody~~, ZV-~~48, bound to ZIKA envelope DIII~~

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

[[Category: ~~Unreleased Structures~~]]

</div>

[[Category: Fremont, D.H]]

== References ==

__TOC__

</StructureSection>

[[Category: Mus musculus]]

[[Category: Structural genomic]]

[[Category: Fremont, D H]]

[[Category: Nelson, C A]]

[[Category: Zhao, H]]

[[Category: ~~Center For Structural Genomics Of Infectious Diseases (~~Csgid)]]

[[Category: Csgid]]

[[Category: ~~Nelson, C.A~~]]

[[Category: Envelope protein]]

[[Category: Single-chain variable fragment]]

[[Category: Viral protein]]

[[Category: Viral protein-immune system complex]]

[[Category: Zika virus]]

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 5kve is ON HOLD
+==Zika specific antibody, ZV-48, bound to ZIKA envelope DIII==
+<StructureSection load='5kve' size='340' side='right' caption='[[5kve]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5kve]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KVE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KVE FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5kvd|5kvd]], [[5kvf|5kvf]], [[5kvg|5kvg]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kve FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kve OCA], [http://pdbe.org/5kve PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kve RCSB], [http://www.ebi.ac.uk/pdbsum/5kve PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kve ProSAT]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Zika virus (ZIKV) infection during pregnancy has emerged as a global public health problem because of its ability to cause severe congenital disease. Here, we developed six mouse monoclonal antibodies (mAbs) against ZIKV including four (ZV-48, ZV-54, ZV-64, and ZV-67) that were ZIKV specific and neutralized infection of African, Asian, and American strains to varying degrees. X-ray crystallographic and competition binding analyses of Fab fragments and scFvs defined three spatially distinct epitopes in DIII of the envelope protein corresponding to the lateral ridge (ZV-54 and ZV-67), C-C' loop (ZV-48 and ZV-64), and ABDE sheet (ZV-2) regions. In vivo passive transfer studies revealed protective activity of DIII-lateral ridge specific neutralizing mAbs in a mouse model of ZIKV infection. Our results suggest that DIII is targeted by multiple type-specific antibodies with distinct neutralizing activity, which provides a path for developing prophylactic antibodies for use in pregnancy or designing epitope-specific vaccines against ZIKV.
-Authors: Zhao, H., Nelson, C.A., Fremont, D.H., Center for Structural Genomics of Infectious Diseases (CSGID)
+Structural Basis of Zika Virus-Specific Antibody Protection.,Zhao H, Fernandez E, Dowd KA, Speer SD, Platt DJ, Gorman MJ, Govero J, Nelson CA, Pierson TC, Diamond MS, Fremont DH Cell. 2016 Jul 26. pii: S0092-8674(16)30927-8. doi: 10.1016/j.cell.2016.07.020. PMID:27475895<ref>PMID:27475895</ref>
-Description: Zika specific antibody, ZV-48, bound to ZIKA envelope DIII
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Fremont, D.H]]
+<div class="pdbe-citations 5kve" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Mus musculus]]
+[[Category: Structural genomic]]
+[[Category: Fremont, D H]]
+[[Category: Nelson, C A]]
 [[Category: Zhao, H]]
-[[Category: Center For Structural Genomics Of Infectious Diseases (Csgid)]]
+[[Category: Csgid]]
-[[Category: Nelson, C.A]]
+[[Category: Envelope protein]]
+[[Category: Single-chain variable fragment]]
+[[Category: Viral protein]]
+[[Category: Viral protein-immune system complex]]
+[[Category: Zika virus]]