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'''Dimeric Structure of the O. nova Telomere End Binding Protein Alpha Subunit with Bound ssDNA''' | '''Dimeric Structure of the O. nova Telomere End Binding Protein Alpha Subunit with Bound ssDNA''' | ||
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[[Category: Ruggles, J A.]] | [[Category: Ruggles, J A.]] | ||
[[Category: Schultz, S C.]] | [[Category: Schultz, S C.]] | ||
[[Category: | [[Category: Dna-protein interaction]] | ||
[[Category: | [[Category: Single stranded dna]] | ||
[[Category: | [[Category: Ssdna]] | ||
[[Category: | [[Category: Telomere binding protein]] | ||
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Revision as of 19:48, 2 May 2008
Dimeric Structure of the O. nova Telomere End Binding Protein Alpha Subunit with Bound ssDNA
Overview
Telomeres are the specialized protein--DNA complexes that cap and protect the ends of linear eukaryotic chromosomes. The extreme 3' end of the telomeric DNA in Oxytricha nova is bound by a two-subunit sequence-specific and 3' end-specific protein called the telomere end-binding protein (OnTEBP). Here we describe the crystal structure of the alpha-subunit of OnTEBP in complex with T4G4 single-stranded telomeric DNA. This structure shows an (alpha--ssDNA)2 homodimer with a large approximately 7,000 A2 protein--protein interface in which the domains of alpha are rearranged extensively from their positions in the structure of an alpha--beta--ssDNA ternary complex. The (alpha--ssDNA)2 complex can bind two telomeres on opposite sides of the dimer and, thus, acts as a protein mediator of telomere--telomere associations. The structures of the (alpha--ssDNA)2 dimer presented here and the previously described alpha--beta--ssDNA complex demonstrate that OnTEBP forms multiple telomeric complexes that potentially mediate the assembly and disassembly of higher order telomeric structures.
About this Structure
1KIX is a Single protein structure of sequence from Sterkiella nova. Full crystallographic information is available from OCA.
Reference
Dimeric structure of the Oxytricha nova telomere end-binding protein alpha-subunit bound to ssDNA., Peersen OB, Ruggles JA, Schultz SC, Nat Struct Biol. 2002 Mar;9(3):182-7. PMID:11836536 Page seeded by OCA on Fri May 2 22:48:08 2008
