4jr8

Crystal structure of cruxrhodopsin-3 from Haloarcula vallismortis at 2.3 angstrom resolution

spinqualitylabelsall models 4jr8, resolution 2.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4jr8 is a 1 chain structure with sequence from Haloarcula vallismortis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: 22B, RET Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function BACR_HALVA Light-driven chloride pump. Publication Abstract from PubMed Cruxrhodopsin-3 (cR3), a retinylidene protein found in the claret membrane of Haloarcula vallismortis, functions as a light-driven proton pump. In this study, the membrane fusion method was applied to crystallize cR3 into a crystal belonging to space group P321. Diffraction data at 2.1 A resolution show that cR3 forms a trimeric assembly with bacterioruberin bound to the crevice between neighboring subunits. Although the structure of the proton-release pathway is conserved among proton-pumping archaeal rhodopsins, cR3 possesses the following peculiar structural features: 1) The DE loop is long enough to interact with a neighboring subunit, strengthening the trimeric assembly; 2) Three positive charges are distributed at the cytoplasmic end of helix F, affecting the higher order structure of cR3; 3) The cytoplasmic vicinity of retinal is more rigid in cR3 than in bacteriorhodopsin, affecting the early reaction step in the proton-pumping cycle; 4) the cytoplasmic part of helix E is greatly bent, influencing the proton uptake process. Meanwhile, it was observed that the photobleaching of retinal, which scarcely occurred in the membrane state, became significant when the trimeric assembly of cR3 was dissociated into monomers in the presence of an excess amount of detergent. On the basis of these observations, we discuss structural factors affecting the photostabilities of ion-pumping rhodopsins. Crystal structure of Cruxrhodopsin-3 from Haloarcula vallismortis.,Chan SK, Kitajima-Ihara T, Fujii R, Gotoh T, Murakami M, Ihara K, Kouyama T PLoS One. 2014 Sep 30;9(9):e108362. doi: 10.1371/journal.pone.0108362., eCollection 2014. PMID:25268964 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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