4k28

2.15 Angstrom resolution crystal structure of a shikimate dehydrogenase family protein from Pseudomonas putida KT2440 in complex with NAD+

spinqualitylabelsall models 4k28, resolution 2.15Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4k28 is a 2 chain structure with sequence from Pseudomonas putida KT2440. This structure supersedes the now removed PDB entry 3tum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: MN, NA, NAD Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q88JP1_PSEPK Publication Abstract from PubMed The shikimate dehydrogenase (SDH) family consists of enzymes with diverse roles in secondary metabolism. The two most widespread members of the family, AroE and YdiB, function in amino acid biosynthesis and quinate catabolism, respectively. Here, we have determined the crystal structure of an SDH homolog belonging to the RifI class, a group of enzymes with proposed roles in antibiotic biosynthesis. The structure of RifI2 from Pseudomonas putida exhibits a number of distinctive features, including a substantial C-terminal truncation and an atypical mode of oligomerization. The active site of the enzyme contains substrate- and cofactor-binding motifs that are significantly different from those of any previously characterized member of the SDH family. These features are reflected in the novel kinetic properties of the enzyme. RifI2 exhibits much lower activity using shikimate as a substrate than AroE, and a strong preference for NAD(+) instead of NADP(+) as a cofactor. Moreover, the enzyme has only trace activity using quinate, unlike YdiB. Cocrystallization of RifI2 with NAD(+) provided the opportunity to determine the mode of cofactor selectivity employed by the enzyme. We complemented this analysis by probing the role of a strictly conserved residue in the cofactor-binding domain, Asn193, by site directed mutagenesis. This study presents the first crystal structure and formal kinetic characterization of a new NAD(+)-dependent member of the SDH family. Insights into the function of RifI2: Structural and biochemical investigation of a new shikimate dehydrogenase family protein.,Peek J, Garcia C, Lee J, Christendat D Biochim Biophys Acta. 2012 Nov 7. pii: S1570-9639(12)00257-9. doi:, 10.1016/j.bbapap.2012.10.016. PMID:23142411 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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