1kpf: Difference between revisions
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'''PKCI-SUBSTRATE ANALOG''' | '''PKCI-SUBSTRATE ANALOG''' | ||
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[[Category: Klein, M G.]] | [[Category: Klein, M G.]] | ||
[[Category: Lima, C D.]] | [[Category: Lima, C D.]] | ||
[[Category: | [[Category: Histidine triad protein family]] | ||
[[Category: | [[Category: Hit protein family]] | ||
[[Category: | [[Category: Nucleotidyl hydrolase]] | ||
[[Category: | [[Category: Nucleotidyl transferase]] | ||
[[Category: | [[Category: Pkci-1]] | ||
[[Category: | [[Category: Protein kinase inhibitor]] | ||
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Revision as of 20:00, 2 May 2008
PKCI-SUBSTRATE ANALOG
Overview
The histidine triad (HIT) protein family is among the most ubiquitous and highly conserved in nature, but a biological activity has not yet been identified for any member of the HIT family. Fragile histidine triad protein (FHIT) and protein kinase C interacting protein (PKCI) were used in a structure-based approach to elucidate characteristics of in vivo ligands and reactions. Crystallographic structures of apo, substrate analog, pentacovalent transition-state analog, and product states of both enzymes reveal a catalytic mechanism and define substrate characteristics required for catalysis, thus unifying the HIT family as nucleotidyl hydrolases, transferases, or both. The approach described here may be useful in identifying structure-function relations between protein families identified through genomics.
About this Structure
1KPF is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure-based analysis of catalysis and substrate definition in the HIT protein family., Lima CD, Klein MG, Hendrickson WA, Science. 1997 Oct 10;278(5336):286-90. PMID:9323207 Page seeded by OCA on Fri May 2 23:00:50 2008
