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'''Crystal Structure of the Acyl-homoserine Lactone Synthase, EsaI''' | '''Crystal Structure of the Acyl-homoserine Lactone Synthase, EsaI''' | ||
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[[Category: Val, D L.]] | [[Category: Val, D L.]] | ||
[[Category: Watson, W T.]] | [[Category: Watson, W T.]] | ||
[[Category: | [[Category: Acylhomoserine lactone]] | ||
[[Category: | [[Category: Alpha-beta]] | ||
[[Category: | [[Category: Autoinducer synthase]] | ||
[[Category: | [[Category: Bacterial pathogenesis]] | ||
[[Category: | [[Category: Quorum sensing]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:21:43 2008'' | |||
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Revision as of 20:21, 2 May 2008
Crystal Structure of the Acyl-homoserine Lactone Synthase, EsaI
Overview
Synthesis and detection of acyl-homoserine lactones (AHLs) enables many gram-negative bacteria to engage in quorum sensing, an intercellular signaling mechanism that activates differentiation to virulent and biofilm lifestyles. The AHL synthases catalyze acylation of S-adenosyl-L-methionine by acyl-acyl carrier protein and lactonization of the methionine moiety to give AHLs. The crystal structure of the AHL synthase, EsaI, determined at 1.8 A resolution, reveals a remarkable structural similarity to the N-acetyltransferases and defines a common phosphopantetheine binding fold as the catalytic core. Critical residues responsible for catalysis and acyl chain specificity have been identified from a modeled substrate complex and verified through functional analysis in vivo. A mechanism for the N-acylation of S-adenosyl-L-methionine by 3-oxo-hexanoyl-acyl carrier protein is proposed.
About this Structure
1KZF is a Single protein structure of sequence from Pantoea stewartii subsp. stewartii. Full crystallographic information is available from OCA.
Reference
Structural basis and specificity of acyl-homoserine lactone signal production in bacterial quorum sensing., Watson WT, Minogue TD, Val DL, von Bodman SB, Churchill ME, Mol Cell. 2002 Mar;9(3):685-94. PMID:11931774 Page seeded by OCA on Fri May 2 23:21:43 2008
