3qp6

Crystal structure of CviR (Chromobacterium violaceum 12472) bound to C6-HSL

spinqualitylabelsall models 3qp6, resolution 2.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3qp6 is a 1 chain structure with sequence from "chromobacterium_janthinum"_(zopf)_ford_1927 "chromobacterium janthinum" (zopf) ford 1927. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: HL6 Related: 3qp1, 3qp2, 3qp4, 3qp5, 3qp7, 3qp8 Gene: cviR ("Chromobacterium janthinum" (Zopf) Ford 1927) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Quorum-sensing bacteria communicate via small molecules called autoinducers to coordinate collective behaviors. Because quorum sensing controls virulence factor expression in many clinically relevant pathogens, membrane-permeable quorum sensing antagonists that prevent population-wide expression of virulence genes offer a potential route to novel antibacterial therapeutics. Here, we report a strategy for inhibiting quorum-sensing receptors of the widespread LuxR family. Structure-function studies with natural and synthetic ligands demonstrate that the dimeric LuxR-type transcription factor CviR from Chromobacterium violaceum is potently antagonized by molecules that bind in place of the native acylated homoserine lactone autoinducer, provided that they stabilize a closed conformation. In such conformations, each of the two DNA-binding domains interacts with the ligand-binding domain of the opposing monomer. Consequently, the DNA-binding helices are held apart by approximately 60 A, twice the approximately 30 A separation required for operator binding. This approach may represent a general strategy for the inhibition of multidomain proteins. A strategy for antagonizing quorum sensing.,Chen G, Swem LR, Swem DL, Stauff DL, O'Loughlin CT, Jeffrey PD, Bassler BL, Hughson FM Mol Cell. 2011 Apr 22;42(2):199-209. PMID:21504831 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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