4q65

Structure of the E. coli Peptide Transporter YbgH

spinqualitylabelsall models 4q65, resolution 3.40Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4q65 is a 1 chain structure with sequence from Ecoli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Gene: dtpD, ybgH, b0709, JW0699 (ECOLI) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [DTPD_ECOLI] Probable proton-dependent permease that transports dipeptides (By similarity). Publication Abstract from PubMed E. coli YbgH belongs to the family of proton-dependent oligopeptide transporters (POTs), a subfamily of the major facilitator superfamily (MFS) of secondary active transporters. Like other MFS transporters, POT proteins switch between two major conformations during substrate transport. Apart from possessing a canonical 12-helix, two-domain transmembrane (TM) core, prokaryotic POT proteins usually have two TM helices inserted between the two domains. Here we determined the crystal structure of YbgH in its inward-facing conformation. Our structure-based functional studies investigated the roles of both the POT signature motif 2 and the inserted interdomain TM helix pair in the stabilization and regulation of the major conformational change in MFS/POT transporters. Furthermore, of all the proton-titratable amino acid residues, Glu21 is the only conserved one (among POTs) located in the central cavity and is critical for in vivo transport. Together, our results support the notion that MFS symporters utilize a transport mechanism based on substrate-protonation coupling. Crystal Structure of the E. coli Peptide Transporter YbgH.,Zhao Y, Mao G, Liu M, Zhang L, Wang X, Zhang XC Structure. 2014 Aug 5;22(8):1152-60. doi: 10.1016/j.str.2014.06.008. Epub 2014, Jul 24. PMID:25066136 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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