1lka: Difference between revisions
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'''Porcine Pancreatic Elastase/Ca-Complex''' | '''Porcine Pancreatic Elastase/Ca-Complex''' | ||
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[[Category: Tucker, P A.]] | [[Category: Tucker, P A.]] | ||
[[Category: Weiss, M S.]] | [[Category: Weiss, M S.]] | ||
[[Category: | [[Category: Acetate binding]] | ||
[[Category: | [[Category: Beta-barrel]] | ||
[[Category: | [[Category: Calcium binding]] | ||
[[Category: | [[Category: Chloride binding]] | ||
[[Category: | [[Category: Chymotrypsin-fold]] | ||
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Revision as of 21:00, 2 May 2008
Porcine Pancreatic Elastase/Ca-Complex
Overview
Porcine pancreatic elastase has been crystallized at slightly acidic pH under two similar but slightly different conditions. Diffraction data were collected at a wavelength of 1.5 A to a maximum resolution of 1.7 A. Both difference electron-density maps and anomalous difference electron-density maps suggest that in crystals grown from a sodium sulfate solution PPE binds Na(+) in its metal-binding site. In contrast, PPE binds Ca(2+) in crystals grown from a solution containing sodium citrate and calcium chloride. This observation is in contradiction to most PPE structures reported in the PDB. In addition to the metal-binding site, up to three other binding sites, which appear to be anion-binding sites, could be identified based on the observed anomalous intensity differences.
About this Structure
1LKA is a Single protein structure of sequence from Sus scrofa. Full crystallographic information is available from OCA.
Reference
Metal binding to porcine pancreatic elastase: calcium or not calcium., Weiss MS, Panjikar S, Nowak E, Tucker PA, Acta Crystallogr D Biol Crystallogr. 2002 Sep;58(Pt 9):1407-12. Epub 2002, Aug 23. PMID:12198296 Page seeded by OCA on Sat May 3 00:00:12 2008
