4c7r

Inward facing conformation of the trimeric betaine transporter BetP in complex with lipids

spinqualitylabelsall models 4c7r, resolution 2.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4c7r is a 3 chain structure with sequence from "micrococcus_glutamicus"_kinoshita_et_al._1958 "micrococcus glutamicus" kinoshita et al. 1958. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CL, CM5, FLC, PEG, PGT Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [BETP_CORGL] High-affinity uptake of glycine betaine (By similarity). Publication Abstract from PubMed Bilayer lipids contribute to the stability of membrane transporters and are crucially involved in their proper functioning. However, the molecular knowledge of how surrounding lipids affect membrane transport is surprisingly limited and despite its general importance is rarely considered in the molecular description of a transport mechanism. One reason is that only few atomic resolution structures of channels or transporters reveal a functional interaction with lipids, which are difficult to detect in X-ray structures per se. Overcoming these difficulties, we report here on a new structure of the osmotic stress-regulated betaine transporter BetP in complex with anionic lipids. This lipid-associated BetP structure is important in the molecular understanding of osmoregulation due to the strong dependence of activity regulation in BetP on the presence of negatively charged lipids. We detected eight resolved palmitoyl-oleoyl phosphatidyl glycerol (PG) lipids mimicking parts of the membrane leaflets and interacting with key residues in transport and regulation. The lipid-protein interactions observed here in structural detail in BetP provide molecular insights into the role of lipids in osmoregulated secondary transport. Structural evidence for functional lipid interactions in the betaine transporter BetP.,Koshy C, Schweikhard ES, Gartner RM, Perez C, Yildiz O, Ziegler C EMBO J. 2013 Oct 18. doi: 10.1038/emboj.2013.226. PMID:24141878 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

Proteopedia Page Contributors and Editors (what is this?)

OCA