3ha4

Crystal structure of the type one membrane protein MIX1 from Leishmania

spinqualitylabelsall models 3ha4, resolution 2.40Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3ha4 is a 8 chain structure with sequence from Leima. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CO3 Gene: LmjF08.1200 (LEIMA) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Infection by Leishmania and Trypanosoma causes severe disease and can be fatal. The reduced effectiveness of current treatments is largely due to drug resistance, hence the urgent need to develop new drugs, preferably against novel targets. We have recently identified a mitochondrial membrane-anchored protein, designated MIX, which occurs exclusively in these parasites and is essential for virulence. We have determined the crystal structure of Leishmania major MIX to a resolution of 2.4 A. MIX forms an all alpha-helical fold comprising seven alpha-helices that fold into a single domain. The distribution of helices is similar to a number of scaffold proteins, namely HEAT repeats, 14-3-3, and tetratricopeptide repeat proteins, suggesting that MIX mediates protein-protein interactions. Accordingly, using copurification and mass spectroscopy we were able to identify several proteins that may interact with MIX in vivo. Being parasite specific, MIX is a promising new drug target and, thus, the structure and potential interacting partners provide a basis for structure-guided drug discovery. Crystal structure of the Leishmania major MIX protein: A scaffold protein that mediates protein-protein interactions.,Gorman MA, Uboldi AD, Walsh PJ, Tan KS, Hansen G, Huyton T, Ji H, Curtis J, Kedzierski L, Papenfuss AT, Dogovski C, Perugini MA, Simpson RJ, Handman E, Parker MW Protein Sci. 2011 Jun;20(6):1060-8. doi: 10.1002/pro.631. Epub 2011 Apr, 27. PMID:21465610 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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