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'''Cholesterol Esterase (Candida Cylindracea) Crystal Structure at 1.4A resolution''' | '''Cholesterol Esterase (Candida Cylindracea) Crystal Structure at 1.4A resolution''' | ||
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[[Category: Pletnev, V.]] | [[Category: Pletnev, V.]] | ||
[[Category: Wawrzak, Z.]] | [[Category: Wawrzak, Z.]] | ||
[[Category: | [[Category: Candida cylindracea cholesterol esterase]] | ||
[[Category: | [[Category: Crystal structure]] | ||
[[Category: | [[Category: Sterol ester acylhydrolase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:02:06 2008'' | |||
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Revision as of 21:02, 2 May 2008
Cholesterol Esterase (Candida Cylindracea) Crystal Structure at 1.4A resolution
Overview
The three-dimensional structure of a Candida cylindracea cholesterol esterase (ChE) homodimer (534 x 2 amino acids) in complex with a ligand of proposed formula C(23)H(48)O(2) has been determined at 1.4 A resolution in space group P1 using synchrotron low-temperature data. The structure refined to R = 0.136 and R(free) = 0.169 and has revealed new stereochemical details in addition to those detected for the apo- and holo-forms at 1.9 and 2.0 A resolution, respectively [Ghosh et al. (1995), Structure, 3, 279-288]. The cholesterol esterase structure is a dimer with four spatially separated interfacial contact areas and two symmetry-related pairs of openings to an internal intradimer cavity. Hydrophobic active-site gorges in each subunit face each other across a central interfacial cavity. The ChE subunits have carbohydrate chains attached to their Asn314 and Asn351 residues, with two ordered N-acetyl-D-glucosoamine moieties visible at each site. The side chains of 14 residues have two alternative conformations with occupancy values of 0.5 +/- 0.2. For each subunit the electron density in the enzyme active-site gorge is well modeled by a C(23)-chain fatty acid.
About this Structure
1LLF is a Single protein structure of sequence from Candida cylindracea. Full crystallographic information is available from OCA.
Reference
Three-dimensional structure of homodimeric cholesterol esterase-ligand complex at 1.4 A resolution., Pletnev V, Addlagatta A, Wawrzak Z, Duax W, Acta Crystallogr D Biol Crystallogr. 2003 Jan;59(Pt 1):50-6. Epub 2002 Dec, 19. PMID:12499539 Page seeded by OCA on Sat May 3 00:02:06 2008
