4ijy: Difference between revisions

Revision as of 20:51, 16 November 2022

Crystal Structure of the ETEC Secreted Protein CofJ

Structural highlights

4ijy is a 1 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	GOL, IOD
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q93I65_ECOLX

Publication Abstract from PubMed

Enterotoxigenic Escherichia coli (ETEC) colonize the human gut, causing severe cholera-like diarrhoea. ETEC utilize a diverse array of pili and fimbriae for host colonization, including the Type IVb pilus CFA/III. The CFA/III pilus machinery is encoded on the cof operon, which is similar in gene sequence and synteny to the tcp operon that encodes another Type IVb pilus, the Vibrio cholerae toxin co-regulated pilus (TCP). Both pilus operons possess a syntenic gene encoding a protein of unknown function. In V. cholerae, this protein, TcpF, is a critical colonization factor secreted by the TCP apparatus. Here we show that the corresponding ETEC protein, CofJ, is a soluble protein secreted via the CFA/III apparatus. We present a 2.6 A resolution crystal structure of CofJ, revealing a large beta-sandwich protein that bears no sequence or structural homology to TcpF. CofJ has a cluster of exposed hydrophobic side-chains at one end and structural homology to the pore-forming proteins perfringolysin O and alpha-haemolysin. CofJ binds to lipid vesicles and epithelial cells, suggesting a role in membrane attachment during ETEC colonization.

Structure and secretion of CofJ, a putative colonization factor of enterotoxigenic Escherichia coli.,Yuen AS, Kolappan S, Ng D, Craig L Mol Microbiol. 2013 Sep 24. doi: 10.1111/mmi.12407. PMID:24106767^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Yuen AS, Kolappan S, Ng D, Craig L. Structure and secretion of CofJ, a putative colonization factor of enterotoxigenic Escherichia coli. Mol Microbiol. 2013 Sep 24. doi: 10.1111/mmi.12407. PMID:24106767 doi:https://dx.doi.org/10.1111/mmi.12407

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OCA

[1] Yuen AS, Kolappan S, Ng D, Craig L. Structure and secretion of CofJ, a putative colonization factor of enterotoxigenic Escherichia coli. Mol Microbiol. 2013 Sep 24. doi: 10.1111/mmi.12407. PMID:24106767 doi:https://dx.doi.org/10.1111/mmi.12407

[1]

@@ Line 1: / Line 1: @@
 ==Crystal Structure of the ETEC Secreted Protein CofJ==
-<StructureSection load='4ijy' size='340' side='right' caption='[[4ijy]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
+<StructureSection load='4ijy' size='340' side='right'caption='[[4ijy]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4ijy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IJY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IJY FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4ijy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IJY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4IJY FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cofJ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ijy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ijy OCA], [https://pdbe.org/4ijy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ijy RCSB], [https://www.ebi.ac.uk/pdbsum/4ijy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ijy ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ijy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ijy OCA], [http://pdbe.org/4ijy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ijy RCSB], [http://www.ebi.ac.uk/pdbsum/4ijy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ijy ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q93I65_ECOLX Q93I65_ECOLX]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Bacillus coli migula 1895]]
+[[Category: Escherichia coli]]
-[[Category: Craig, L]]
+[[Category: Large Structures]]
-[[Category: Kolappan, S]]
+[[Category: Craig L]]
-[[Category: Yuen, A S.W]]
+[[Category: Kolappan S]]
-[[Category: Beta-sandwich]]
+[[Category: Yuen ASW]]
-[[Category: Secreted protein]]
-[[Category: Unknown function]]

4ijy: Difference between revisions

Revision as of 20:51, 16 November 2022

Crystal Structure of the ETEC Secreted Protein CofJ

Structural highlights

Function

Publication Abstract from PubMed

References

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