4kr5: Difference between revisions

Revision as of 09:08, 7 December 2022

Crystal structure of Lactococcus lactis GlnP substrate binding domain 2 (SBD2) in open conformation

Structural highlights

4kr5 is a 2 chain structure with sequence from Lactococcus lactis subsp. lactis Il1403. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	CL, MG, P33, P6G, PE4, PEG, PG0
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q9CES5_LACLA

Publication Abstract from PubMed

The ATP-binding cassette (ABC) transporter GlnPQ is an essential uptake system for amino acids in gram-positive pathogens and related nonpathogenic bacteria. The transporter has tandem substrate-binding domains (SBDs) fused to each transmembrane domain, giving rise to four SBDs per functional transporter complex. We have determined the crystal structures and ligand-binding properties of the SBDs of GlnPQ from Enterococcus faecalis, Streptococcus pneumoniae, and Lactococcus lactis. The tandem SBDs differ in substrate specificity and affinity, allowing cells to efficiently accumulate different amino acids via a single ABC transporter. The combined structural, functional, and thermodynamic analysis revealed the roles of individual residues in determining the substrate affinity. We succeeded in converting a low-affinity SBD into a high-affinity receptor and vice versa. Our data indicate that a small number of residues that reside in the binding pocket constitute the major affinity determinants of the SBDs.

Functional Diversity of Tandem Substrate-Binding Domains in ABC Transporters from Pathogenic Bacteria.,Fulyani F, Schuurman-Wolters GK, Zagar AV, Guskov A, Slotboom DJ, Poolman B Structure. 2013 Aug 28. pii: S0969-2126(13)00270-0. doi:, 10.1016/j.str.2013.07.020. PMID:23994008^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Fulyani F, Schuurman-Wolters GK, Zagar AV, Guskov A, Slotboom DJ, Poolman B. Functional Diversity of Tandem Substrate-Binding Domains in ABC Transporters from Pathogenic Bacteria. Structure. 2013 Aug 28. pii: S0969-2126(13)00270-0. doi:, 10.1016/j.str.2013.07.020. PMID:23994008 doi:10.1016/j.str.2013.07.020

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Fulyani F, Schuurman-Wolters GK, Zagar AV, Guskov A, Slotboom DJ, Poolman B. Functional Diversity of Tandem Substrate-Binding Domains in ABC Transporters from Pathogenic Bacteria. Structure. 2013 Aug 28. pii: S0969-2126(13)00270-0. doi:, 10.1016/j.str.2013.07.020. PMID:23994008 doi:10.1016/j.str.2013.07.020

[1]

@@ Line 1: / Line 1: @@
 ==Crystal structure of Lactococcus lactis GlnP substrate binding domain 2 (SBD2) in open conformation==
-<StructureSection load='4kr5' size='340' side='right' caption='[[4kr5]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
+<StructureSection load='4kr5' size='340' side='right'caption='[[4kr5]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4kr5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lacla Lacla]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KR5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KR5 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4kr5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactococcus_lactis_subsp._lactis_Il1403 Lactococcus lactis subsp. lactis Il1403]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KR5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KR5 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=P33:3,6,9,12,15,18-HEXAOXAICOSANE-1,20-DIOL'>P33</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=PE4:2-{2-[2-(2-{2-[2-(2-ETHOXY-ETHOXY)-ETHOXY]-ETHOXY}-ETHOXY)-ETHOXY]-ETHOXY}-ETHANOL'>PE4</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG0:2-(2-METHOXYETHOXY)ETHANOL'>PG0</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=P33:3,6,9,12,15,18-HEXAOXAICOSANE-1,20-DIOL'>P33</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=PE4:2-{2-[2-(2-{2-[2-(2-ETHOXY-ETHOXY)-ETHOXY]-ETHOXY}-ETHOXY)-ETHOXY]-ETHOXY}-ETHANOL'>PE4</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG0:2-(2-METHOXYETHOXY)ETHANOL'>PG0</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4kqp|4kqp]], [[4kpt|4kpt]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kr5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kr5 OCA], [https://pdbe.org/4kr5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kr5 RCSB], [https://www.ebi.ac.uk/pdbsum/4kr5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kr5 ProSAT]</span></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">glnP, L165, LL1759 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272623 LACLA])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kr5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kr5 OCA], [http://pdbe.org/4kr5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4kr5 RCSB], [http://www.ebi.ac.uk/pdbsum/4kr5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4kr5 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q9CES5_LACLA Q9CES5_LACLA]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 20: / Line 20: @@
 ==See Also==
-*[[ABC transporter|ABC transporter]]
+*[[ABC transporter 3D structures|ABC transporter 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Lacla]]
+[[Category: Lactococcus lactis subsp. lactis Il1403]]
-[[Category: Guskov, A]]
+[[Category: Large Structures]]
-[[Category: Poolman, B]]
+[[Category: Guskov A]]
-[[Category: Schuurman-Wolters, G K]]
+[[Category: Poolman B]]
-[[Category: Slotboom, D J]]
+[[Category: Schuurman-Wolters GK]]
-[[Category: Zagar, A Vujicic]]
+[[Category: Slotboom DJ]]
-[[Category: Amino acid transport]]
+[[Category: Vujicic Zagar A]]
-[[Category: Extracellular]]
-[[Category: Glutamine/glutamic acid binding]]
-[[Category: Transport protein]]

4kr5: Difference between revisions

Revision as of 09:08, 7 December 2022

Crystal structure of Lactococcus lactis GlnP substrate binding domain 2 (SBD2) in open conformation

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools