1b5g: Difference between revisions
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==Overview== | ==Overview== | ||
The X-ray crystal structures of four beta-strand-templated active site, inhibitors of thrombin containing P1' groups have been determined and, refined at about 2.1-A resolution to crystallographic R-values between, 0.148 and 0.164. Two of the inhibitors have an alpha-ketoamide, functionality at the scissile bond; the other two have a nonhydrolyzable, electrophilic group at the P1' position. The binding of lysine is compared, with that of arginine at the S1 specificity site, while that of, D,L-phenylalanine enantiomorphs is compared in the S3 region of thrombin., Four different P1' moieties bind at the S1' subsite in three different, ways. The binding constants vary between 2.0 microM and 70 pM. The bound, structures are used to intercorrelate the various binding constants and, also lead to insightful inferences concerning binding at the S1' site of, thrombin. | The X-ray crystal structures of four beta-strand-templated active site, inhibitors of thrombin containing P1' groups have been determined and, refined at about 2.1-A resolution to crystallographic R-values between, 0.148 and 0.164. Two of the inhibitors have an alpha-ketoamide, functionality at the scissile bond; the other two have a nonhydrolyzable, electrophilic group at the P1' position. The binding of lysine is compared, with that of arginine at the S1 specificity site, while that of, D,L-phenylalanine enantiomorphs is compared in the S3 region of thrombin., Four different P1' moieties bind at the S1' subsite in three different, ways. The binding constants vary between 2.0 microM and 70 pM. The bound, structures are used to intercorrelate the various binding constants and, also lead to insightful inferences concerning binding at the S1' site of, thrombin. | ||
==Disease== | |||
Known diseases associated with this structure: Dysprothrombinemia OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=176930 176930]], Hyperprothrombinemia OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=176930 176930]], Hypoprothrombinemia OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=176930 176930]] | |||
==About this Structure== | ==About this Structure== | ||
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[[Category: complex (serine protease/inhibitor)]] | [[Category: complex (serine protease/inhibitor)]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 16:06:03 2007'' | ||
