Structural highlights
3knr is a 4 chain structure with sequence from Atcc 14579. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
|
| Ligands: | ACY, GOL, SO4, ZN |
| Related: | 1bvt, 1bc2, 3kns |
| Gene: | blm (ATCC 14579) |
| Activity: | Beta-lactamase, with EC number 3.5.2.6 |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
[BLA2_BACCE] Can hydrolyze carbapenem compounds.
Publication Abstract from PubMed
A number of multiresistant bacterial pathogens inactivate antibiotics by producing Zn(II)-dependent beta-lactamases. We show that metal uptake leading to an active dinuclear enzyme in the periplasmic space of Gram-negative bacteria is ensured by a cysteine residue, an unusual metal ligand in oxidizing environments. Kinetic, structural and affinity data show that such Zn(II)-cysteine interaction is an adaptive trait that tunes the metal binding affinity, thus enabling antibiotic resistance at restrictive Zn(II) concentrations.
Metallo-beta-lactamases withstand low Zn(II) conditions by tuning metal-ligand interactions.,Gonzalez JM, Meini MR, Tomatis PE, Martin FJ, Cricco JA, Vila AJ Nat Chem Biol. 2012 Jun 24. doi: 10.1038/nchembio.1005. PMID:22729148[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Gonzalez JM, Meini MR, Tomatis PE, Martin FJ, Cricco JA, Vila AJ. Metallo-beta-lactamases withstand low Zn(II) conditions by tuning metal-ligand interactions. Nat Chem Biol. 2012 Jun 24. doi: 10.1038/nchembio.1005. PMID:22729148 doi:10.1038/nchembio.1005
