4bff

Superoxide reductase (Neelaredoxin) from Archaeoglobus fulgidus in the reduced form

spinqualitylabelsall models 4bff, resolution 2.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4bff is a 16 chain structure with sequence from Arcfl. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: FE2 Related: 4bfj, 4bfk Activity: Superoxide reductase, with EC number 1.15.1.2 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [SOR_ARCFU] Uses electrons from reduced NADP, by way of rubredoxin and an oxidoreductase, to catalyze the reduction of superoxide to hydrogen peroxide (By similarity). Publication Abstract from PubMed Neelaredoxins are a type of superoxide reductase (SOR), which are blue 14 kDa metalloproteins with a catalytic nonhaem iron centre coordinated by four histidines and one cysteine in the ferrous form. Anaerobic organisms such as Archaeoglobus fulgidus, a hyperthermophilic sulfate-reducing archaeon, have developed defence mechanisms against toxic oxygen species in which superoxide reductases play a key role. SOR is responsible for scavenging toxic superoxide anion radicals (O(2)(*-)), catalysing the one-electron reduction of superoxide to hydrogen peroxide. Crystals of recombinant A. fulgidus neelaredoxin in the oxidized form (13.7 kDa, 125 residues) were obtained using polyethylene glycol and ammonium sulfate. These crystals diffracted to 1.9 A resolution and belonged to the tetragonal space group P4(1)2(1)2, with unit-cell parameters a = b = 75.72, c = 185.44 A. Cell-content analysis indicated the presence of a tetramer in the asymmetric unit, with a Matthews coefficient (V(M)) of 2.36 A(3) Da(-1) and an estimated solvent content of 48%. The three-dimensional structure was determined by the MAD method and is currently under refinement. Purification, crystallization and X-ray crystallographic analysis of Archaeoglobus fulgidus neelaredoxin.,Bandeiras TM, Romao CV, Rodrigues JV, Teixeira M, Matias PM Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Mar 1;66(Pt 3):316-9. doi:, 10.1107/S1744309110000916. Epub 2010 Feb 24. PMID:20208170 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Superoxide Reductase

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