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==Crystal structure of Amylosucrase double mutant A289P-F290L from Neisseria polysaccharea==
==Crystal structure of Amylosucrase double mutant A289P-F290L from Neisseria polysaccharea==
<StructureSection load='4flr' size='340' side='right' caption='[[4flr]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
<StructureSection load='4flr' size='340' side='right'caption='[[4flr]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4flr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"neisseria_polysacchareae"_riou_et_al._1983 "neisseria polysacchareae" riou et al. 1983]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FLR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FLR FirstGlance]. <br>
<table><tr><td colspan='2'>[[4flr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Neisseria_polysaccharea Neisseria polysaccharea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FLR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FLR FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1g5a|1g5a]], [[4flo|4flo]], [[4flq|4flq]], [[4fls|4fls]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4flr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4flr OCA], [https://pdbe.org/4flr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4flr RCSB], [https://www.ebi.ac.uk/pdbsum/4flr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4flr ProSAT]</span></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ams ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=489 "Neisseria polysacchareae" Riou et al. 1983])</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Amylosucrase Amylosucrase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.4 2.4.1.4] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4flr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4flr OCA], [http://pdbe.org/4flr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4flr RCSB], [http://www.ebi.ac.uk/pdbsum/4flr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4flr ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/AMYS_NEIPO AMYS_NEIPO]] Catalyzes the synthesis of alpha-glucan from sucrose. Catalyzes, in addition, sucrose hydrolysis, maltose and maltotriose synthesis by successive transfers of the glucosyl moiety of sucrose onto the released glucose, and finally turanose and trehalulose synthesis, these two sucrose isomers being obtained by glucosyl transfer onto fructose.<ref>PMID:9882648</ref>
[https://www.uniprot.org/uniprot/AMYS_NEIPO AMYS_NEIPO] Catalyzes the synthesis of alpha-glucan from sucrose. Catalyzes, in addition, sucrose hydrolysis, maltose and maltotriose synthesis by successive transfers of the glucosyl moiety of sucrose onto the released glucose, and finally turanose and trehalulose synthesis, these two sucrose isomers being obtained by glucosyl transfer onto fructose.<ref>PMID:9882648</ref>  
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Neisseria polysacchareae riou et al. 1983]]
[[Category: Large Structures]]
[[Category: Amylosucrase]]
[[Category: Neisseria polysaccharea]]
[[Category: Andre, I]]
[[Category: Andre I]]
[[Category: Barbe, S]]
[[Category: Barbe S]]
[[Category: Champion, E]]
[[Category: Champion E]]
[[Category: Descroix, K]]
[[Category: Descroix K]]
[[Category: Guerin, F]]
[[Category: Guerin F]]
[[Category: Monsan, P]]
[[Category: Monsan P]]
[[Category: Morel, S]]
[[Category: Morel S]]
[[Category: Moulis, C]]
[[Category: Moulis C]]
[[Category: Mourey, L]]
[[Category: Mourey L]]
[[Category: Mulard, L A]]
[[Category: Mulard LA]]
[[Category: Remaud-Simeon, M]]
[[Category: Remaud-Simeon M]]
[[Category: Tran, T H]]
[[Category: Tran TH]]
[[Category: Tranier, S]]
[[Category: Tranier S]]
[[Category: Amylose synthesis]]
[[Category: Beta/alpha-barrel]]
[[Category: Carbohydrate]]
[[Category: Glucosyltransferase]]
[[Category: Glycoside hydrolase]]
[[Category: Sucrose isomerization]]
[[Category: Transferase]]
Retrieved from "https://proteopedia.org/4flr"