1bix: Difference between revisions
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==Overview== | ==Overview== | ||
The structure of the major human apurinic/ apyrimidinic endonuclease, (HAP1) has been solved at 2.2 A resolution. The enzyme consists of two, symmetrically related domains of similar topology and has significant, structural similarity to both bovine DNase I and its Escherichia coli, homologue exonuclease III (EXOIII). A structural comparison of these, enzymes reveals three loop regions specific to HAP1 and EXOIII. These loop, regions apparently act in DNA abasic site (AP) recognition and cleavage, since DNase I, which lacks these loops, correspondingly lacks AP site, specificity. The HAP1 structure furthermore suggests a mechanism for AP, site binding which involves the recognition of the deoxyribose moiety in, an extrahelical conformation, rather than a 'flipped-out' base opposite, the AP site. | The structure of the major human apurinic/ apyrimidinic endonuclease, (HAP1) has been solved at 2.2 A resolution. The enzyme consists of two, symmetrically related domains of similar topology and has significant, structural similarity to both bovine DNase I and its Escherichia coli, homologue exonuclease III (EXOIII). A structural comparison of these, enzymes reveals three loop regions specific to HAP1 and EXOIII. These loop, regions apparently act in DNA abasic site (AP) recognition and cleavage, since DNase I, which lacks these loops, correspondingly lacks AP site, specificity. The HAP1 structure furthermore suggests a mechanism for AP, site binding which involves the recognition of the deoxyribose moiety in, an extrahelical conformation, rather than a 'flipped-out' base opposite, the AP site. | ||
==Disease== | |||
Known diseases associated with this structure: Autoimmune polyglandular disease, type I OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=607358 607358]], Sveinsson choreoretinal atrophy OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=189967 189967]] | |||
==About this Structure== | ==About this Structure== | ||
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[[Category: ref-1]] | [[Category: ref-1]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 16:10:33 2007'' | ||
