4bwf

Pex4p-Pex22p disulphide bond mutant

spinqualitylabelsall models 4bwf, resolution 3.23Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4bwf is a 2 chain structure with sequence from Atcc 18824. This structure supersedes the now removed PDB entry 2y9o. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: EDO Activity: Ubiquitin--protein ligase, with EC number 6.3.2.19 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [UBCX_YEAST] Catalyzes the covalent attachment of ubiquitin to other proteins. Essential for peroxisome biogenesis. Required for UBC4-independent ubiquitination of PEX5. [PEX22_YEAST] Involved in peroxisome biogenesis (By similarity). Publication Abstract from PubMed The ubiquitin-conjugating enzyme Pex4p together with its binding partner, the peroxisomal membrane protein Pex22p, co-ordinates cysteine-dependent ubiquitination of the cycling receptor protein Pex5p. Unusually for an ubiquitin-conjugating enzyme, Saccharomyces cerevisiae Pex4p can form a disulphide bond between the cysteine residues at positions 105 and 146. We found that mutating the disulphide forming cysteine residues in Pex4p to serines does not disturb the secondary structure of the protein but does reduce the in vitro activity of Pex4p. From the crystal structure of Pex4p C105S, C146S in complex with the soluble domain of Pex22p, we observe a narrowing of the active site cleft, caused by loss of the disulphide bond. This modification of the active site microenvironment is likely to restrict access of ubiquitin to the active site cysteine, modulating Pex4p activity. Finally, based on sequence and structural alignments, we have identified other ubiquitin-conjugating enzymes that may contain disulphide bonds. A disulphide bond in the E2 enzyme Pex4p modulates ubiquitin-conjugating activity.,Williams C, van den Berg M, Stanley WA, Wilmanns M, Distel B Sci Rep. 2013 Jul 30;3:2212. doi: 10.1038/srep02212. PMID:23896733 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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