3zrd

Oxidised Thiol peroxidase (Tpx) from Yersinia pseudotuberculosis

spinqualitylabelsall models 3zrd, resolution 1.74Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3zrd is a 2 chain structure with sequence from Yerpy. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Related: 3zre, 2yjh Activity: Peroxiredoxin, with EC number 1.11.1.15 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [Q66A71_YERPS] Has antioxidant activity. Could remove peroxides or H(2)O(2) (By similarity).[HAMAP-Rule:MF_00269] Publication Abstract from PubMed Thiol peroxidase, Tpx, has been shown to be a target protein of the salicylidene acylhydrazide class of antivirulence compounds. In this study we present the crystal structures of Tpx from Y. pseudotuberculosis (ypTpx) in the oxidised and reduced states, together with the structure of the C61S mutant. The structures solved are consistent with previously solved atypical 2-Cys thiol peroxidases, including that for "forced" reduced states using the C61S mutant. In addition, by investigating the solution structure of ypTpx using small angle X-ray scattering (SAXS), we have confirmed that reduced state ypTpx in solution is a homodimer. The solution structure also reveals flexibility around the dimer interface. Notably, the conformational changes observed between the redox states at the catalytic triad and at the dimer interface have implications for substrate and inhibitor binding. The structural data were used to model the binding of two salicylidene acylhydrazide compounds to the oxidised structure of ypTpx. Overall, the study provides insights into the binding of the salicylidene acylhydrazides to ypTpx, aiding our long-term strategy to understand the mode of action of this class of compounds. Structural Characterisation of Tpx from Yersinia pseudotuberculosis Reveals Insights into the Binding of Salicylidene Acylhydrazide Compounds.,Gabrielsen M, Beckham KS, Feher VA, Zetterstrom CE, Wang D, Muller S, Elofsson M, Amaro RE, Byron O, Roe AJ PLoS One. 2012;7(2):e32217. Epub 2012 Feb 27. PMID:22384182 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Thiol peroxidase

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