3tky

Monolignol o-methyltransferase (momt)

spinqualitylabelsall models 3tky, resolution 2.47Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3tky is a 4 chain structure with sequence from Clabr. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: N7I, SAH Gene: IEMT1 (CLABR) Activity: (Iso)eugenol O-methyltransferase, with EC number 2.1.1.146 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [IEMT_CLABR] Catalyzes the methylation of the para-4-hydroxyl of both eugenol and (iso)eugenol to methyleugenol and isomethyleugenol, respectively. The resulting products are part of a complex mixture of low-molecular-weight volatile compounds emitted by the flowers to attract pollinators. Publication Abstract from PubMed Although the practice of protein engineering is industrially fruitful in creating biocatalysts and therapeutic proteins, applications of analogous techniques in the field of plant metabolic engineering are still in their infancy. Lignins are aromatic natural polymers derived from the oxidative polymerization of primarily three different hydroxycinnamyl alcohols, the monolignols. Polymerization of lignin starts with the oxidation of monolignols, followed by endwise cross-coupling of (radicals of) a monolignol and the growing oligomer/polymer. The para-hydroxyl of each monolignol is crucial for radical generation and subsequent coupling. Here, we describe the structure-function analysis and catalytic improvement of an artificial monolignol 4-O-methyltransferase created by iterative saturation mutagenesis and its use in modulating lignin and phenylpropanoid biosynthesis. We show that expressing the created enzyme in planta, thus etherifying the para-hydroxyls of lignin monomeric precursors, denies the derived monolignols any participation in the subsequent coupling process, substantially reducing lignification and, ultimately, lignin content. Concomitantly, the transgenic plants accumulated de novo synthesized 4-O-methylated soluble phenolics and wall-bound esters. The lower lignin levels of transgenic plants resulted in higher saccharification yields. Our study, through a structure-based protein engineering approach, offers a novel strategy for modulating phenylpropanoid/lignin biosynthesis to improve cell wall digestibility and diversify the repertories of biologically active compounds. An engineered monolignol 4-o-methyltransferase depresses lignin biosynthesis and confers novel metabolic capability in Arabidopsis.,Zhang K, Bhuiya MW, Pazo JR, Miao Y, Kim H, Ralph J, Liu CJ Plant Cell. 2012 Jul;24(7):3135-52. Epub 2012 Jul 31. PMID:22851762 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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