3vw0

Crystal Structure of The Dequalinum-bound Form of RamR (Transcriptional Regulator of TetR Family) From Salmonella Typhimurium

spinqualitylabelsall models 3vw0, resolution 2.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3vw0 is a 4 chain structure with sequence from Salt1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: DEQ, SO4 Related: 3vvx, 3vvy, 3vvz, 3vw1, 3vw2 Gene: STM0580, STM14_0676 (SALT1) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed RamR is a transcriptional repressor of the gene-encoding RamA protein, which controls the expression of the multidrug efflux system genes acrAB-tolC. RamR is an important multidrug-resistance factor, however, its structure and the identity of the molecules to which it responds have been unknown. Here, we report the crystal structure of RamR in complex with multiple drugs, including berberine, crystal violet, dequalinium, ethidium bromide and rhodamine 6G. All compounds are found to interact with Phe155 of RamR, and each compound is surrounded by different amino acid residues. Binding of these compounds to RamR reduces its DNA-binding affinity, which results in the increased expression of ramA. Our results reveal significant flexibility in the substrate-recognition region of RamR, which regulates the bacterial efflux participating in multidrug resistance. The crystal structure of multidrug-resistance regulator RamR with multiple drugs.,Yamasaki S, Nikaido E, Nakashima R, Sakurai K, Fujiwara D, Fujii I, Nishino K Nat Commun. 2013 Jun 26;4:2078. doi: 10.1038/ncomms3078. PMID:23800819 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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