4ena

Crystal structure of fluoride riboswitch, soaked in Cs+

spinqualitylabelsall models 4ena, resolution 2.85Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4ena is a 1 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CS, F, MG NonStd Res: GTP Related: 4en5, 4enb, 4enc, 3vrs Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Significant advances in our understanding of RNA architecture, folding and recognition have emerged from structure-function studies on riboswitches, non-coding RNAs whose sensing domains bind small ligands and whose adjacent expression platforms contain RNA elements involved in the control of gene regulation. We now report on the ligand-bound structure of the Thermotoga petrophila fluoride riboswitch, which adopts a higher-order RNA architecture stabilized by pseudoknot and long-range reversed Watson-Crick and Hoogsteen A*U pair formation. The bound fluoride ion is encapsulated within the junctional architecture, anchored in place through direct coordination to three Mg(2+) ions, which in turn are octahedrally coordinated to water molecules and five inwardly pointing backbone phosphates. Our structure of the fluoride riboswitch in the bound state shows how RNA can form a binding pocket selective for fluoride, while discriminating against larger halide ions. The T. petrophila fluoride riboswitch probably functions in gene regulation through a transcription termination mechanism. Fluoride ion encapsulation by Mg2+ ions and phosphates in a fluoride riboswitch.,Ren A, Rajashankar KR, Patel DJ Nature. 2012 May 13;486(7401):85-9. doi: 10.1038/nature11152. PMID:22678284 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Riboswitch

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