4aps

Crystal structure of a POT family peptide transporter in an inward open conformation.

spinqualitylabelsall models 4aps, resolution 3.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4aps is a 2 chain structure with sequence from Strt2. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CD Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Short chain peptides are actively transported across membranes as an efficient route for dietary protein absorption and for maintaining cellular homeostasis. In mammals, peptide transport occurs via PepT1 and PepT2, which belong to the proton-dependent oligopeptide transporter, or POT family. The recent crystal structure of a bacterial POT transporter confirmed that they belong to the major facilitator superfamily of secondary active transporters. Despite the functional characterization of POT family members in bacteria, fungi and mammals, a detailed model for peptide recognition and transport remains unavailable. In this study, we report the 3.3-A resolution crystal structure and functional characterization of a POT family transporter from the bacterium Streptococcus thermophilus. Crystallized in an inward open conformation the structure identifies a hinge-like movement within the C-terminal half of the transporter that facilitates opening of an intracellular gate controlling access to a central peptide-binding site. Our associated functional data support a model for peptide transport that highlights the importance of salt bridge interactions in orchestrating alternating access within the POT family. Alternating access mechanism in the POT family of oligopeptide transporters.,Solcan N, Kwok J, Fowler PW, Cameron AD, Drew D, Iwata S, Newstead S EMBO J. 2012 Jun 1. doi: 10.1038/emboj.2012.157. PMID:22659829 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Symporter

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