3udc

Crystal structure of a membrane protein

spinqualitylabelsall models 3udc, resolution 3.35Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3udc is a 7 chain structure with sequence from Ecoli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Related: 3t9n Gene: MscS (ECOLI) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Mechanosensitive (MS) channels are universal cellular membrane pores. Bacterial MS channels, as typified by MS channel of small conductance (MscS) from Escherichia coli (EcMscS), release osmolytes under hypoosmotic conditions. MS channels are known to be ion selective to different extents, but the underlying mechanism remains poorly understood. Here we identify an anion-selective MscS channel from Thermoanaerobacter tengcongensis (TtMscS). The structure of TtMscS closely resembles that of EcMscS, but it lacks the large cytoplasmic equatorial portals found in EcMscS. In contrast, the cytoplasmic pore formed by the C-terminal beta-barrel of TtMscS is larger than that of EcMscS and has a strikingly different pattern of electrostatic surface potential. Swapping the beta-barrel region between TtMscS and EcMscS partially switches the ion selectivity. Our study defines the role of the beta-barrel in the ion selection of an anion-selective MscS channel and provides a structural basis for understanding the ion selectivity of MscS channels. Structure and molecular mechanism of an anion-selective mechanosensitive channel of small conductance.,Zhang X, Wang J, Feng Y, Ge J, Li W, Sun W, Iscla I, Yu J, Blount P, Li Y, Yang M Proc Natl Acad Sci U S A. 2012 Oct 30;109(44):18180-5. doi:, 10.1073/pnas.1207977109. Epub 2012 Oct 16. PMID:23074248 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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