3bvq

Crystal Structure of Apo NotI Restriction Endonuclease

spinqualitylabelsall models 3bvq, resolution 2.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3bvq is a 2 chain structure with sequence from "actinomyces_caviae"_erikson_1935 "actinomyces caviae" erikson 1935. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: FE, SO4 NonStd Res: MSE Related: 3bvr Gene: notIR ("Actinomyces caviae" Erikson 1935) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed The structure of the rare-cutting restriction endonuclease NotI, which recognizes the 8 bp target 5'-GCGGCCGC-3', has been solved with and without bound DNA. Because of its specificity (recognizing a site that occurs once per 65 kb), NotI is used to generate large genomic fragments and to map DNA methylation status. NotI contains a unique metal binding fold, found in a variety of putative endonucleases, occupied by an iron atom coordinated within a tetrahedral Cys4 motif. This domain positions nearby protein elements for DNA recognition, and serves a structural role. While recognition of the central six base pairs of the target is accomplished via a saturated hydrogen bond network typical of restriction enzymes, the most peripheral base pairs are engaged in a single direct contact in the major groove, reflecting reduced pressure to recognize those positions. NotI may represent an evolutionary intermediate between mobile endonucleases (which recognize longer target sites) and canonical restriction endonucleases. Structures of the Rare-Cutting Restriction Endonuclease NotI Reveal a Unique Metal Binding Fold Involved in DNA Binding.,Lambert AR, Sussman D, Shen B, Maunus R, Nix J, Samuelson J, Xu SY, Stoddard BL Structure. 2008 Apr;16(4):558-69. PMID:18400177 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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